Name: Anti-LEPREL1 Antibody (CAB8068)
Brand: Cell Biology Antibodies 11
SKU: CAB8068
Availability: OutOfStock

Antibody Name:	LEPREL1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8068
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human LEPREL1 (NP_060662.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human LEPREL1 (NP_060662.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IRYG GRQD ENRV PSGV NVEG AEVH GFSM GKKL SPKI DRDL REGG PLLY ENIT FVYN SEQL NGTQ RVLL DNVL SEEQ CREL HSVA SGIM LVGD GYRG KTSP HTPN EKFE GATV LKAL KSGY EGRV PLKS ARLF YDIS EKAR RIVE SYFM LNST LYFS YTHM VCRT ALSG QQDR RNDL SHPI HADN CLLD PEAN ECWK EPPA YTFR DYSA LLYM NDDF EGGE FIFT EMDA KTVT ASIK PKCG RMIS FSSG GENP HGVK AVTK GKRC AVAL WFTL DPLY RELE RIQA DEVI AILD QEQQ GKHE L
Gene ID:	55214
Uniprot:	Q8IVL5
Cellular Location:	Endoplasmic reticulum, Golgi apparatus
Calculated MW:	60kDa/80kDa
Observed MW:	85kDa

Synonyms:

P3H2, LEPREL1, MCVD, MLAT4

Background:

This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	LEPREL1: Shows prolyl 3-hydroxylase activity catalyzing the post- translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types II, IV and V. Defects in LEPREL1 are the cause of myopia high with cataract and vitreoretinal degeneration (MCVD). A disorder characterized by severe myopia with variable expressivity of cataract and vitreoretinal degeneration. Some patients manifest lens subluxation, lens instability and retinal detachment. Belongs to the leprecan family.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.14.11.7 Chromosomal Location of Human Ortholog: 3q28 Cellular Component: Golgi apparatus; endoplasmic reticulum; endoplasmic reticulum lumen; basement membrane Molecular Function:procollagen-proline 3-dioxygenase activity; L-ascorbic acid binding; iron ion binding Biological Process: negative regulation of cell proliferation; extracellular matrix organization and biogenesis; collagen metabolic process; peptidyl-proline hydroxylation Disease: Myopia, High, With Cataract And Vitreoretinal Degeneration
NCBI Summary:	This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q8IVL5
NCBI GenInfo Identifier:	74714365
NCBI Gene ID:	55214
NCBI Accession:	Q8IVL5.1
UniProt Related Accession:	Q8IVL5
Molecular Weight:
NCBI Full Name:	Prolyl 3-hydroxylase 2
NCBI Synonym Full Names:	prolyl 3-hydroxylase 2
NCBI Official Symbol:	P3H2
NCBI Official Synonym Symbols:	MCVD; MLAT4; LEPREL1
NCBI Protein Information:	prolyl 3-hydroxylase 2
UniProt Protein Name:	Prolyl 3-hydroxylase 2
UniProt Synonym Protein Names:	Leprecan-like protein 1; Myxoid liposarcoma-associated protein 4
Protein Family:	Prolyl 3-hydroxylase
UniProt Gene Name:	LEPREL1
UniProt Entry Name:	P3H2_HUMAN

Anti-LEPREL1 Polyclonal Antibody (CAB8068)

Western blot analysis of extracts of NCI-H460 cells, using LEPREL1 antibody (CAB8068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

LEPREL1 Antibody (PACO10233)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)