Antibody Name:	Anti-LECT1 Antibody
Antibody SKU:	CAB6644
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 75-334 of human LECT1 (NP_008946.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 75-334 of human LECT1 (NP_008946.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VHYT MSIN GKLQ DGSM EIDA GNNL ETFK MGSG AEEA IAVN DFQN GITG IRFA GGEK CYIK AQVK ARIP EVGA VTKQ SISS KLEG KIMP VKYE ENSL IWVA VDQP VKDN SFLS SKVL ELCG DLPI FWLK PTYP KEIQ RERR EVVR KIVP TTTK RPHS GPRS NPGA GRLN NETR PSVQ EDSQ AFNP DNPY HQQE GESM TFDP RLDH EGIC CIEC RRSY THCQ KICE PLGG YYPW PYNY QGCR SACR VIMP CSWW VARI LGMV
Gene ID:	11061
Uniprot:	O75829
Cellular Location:	Endomembrane system, Secreted, Single-pass membrane protein, extracellular matrix, extracellular space
Calculated MW:	36kDa/37kDa
Observed MW:	37kDa

Synonyms:

CNMD, BRICD3, CHM-I, CHM1, LECT1, MYETS1

Background:

This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	LECT1: Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization. Belongs to the chondromodulin-1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell development/differentiation; Membrane protein, integral Chromosomal Location of Human Ortholog: 13q14.3 Biological Process: negative regulation of angiogenesis; proteoglycan metabolic process; skeletal development
NCBI Summary:	This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O75829
NCBI GenInfo Identifier:	18202146
NCBI Gene ID:	11061
NCBI Accession:	O75829.1
UniProt Secondary Accession:	O75829,Q5TAM4, Q8TAY6, Q9UM18,
UniProt Related Accession:	O75829
Molecular Weight:	36,974 Da
NCBI Full Name:	Leukocyte cell-derived chemotaxin 1
NCBI Synonym Full Names:	leukocyte cell derived chemotaxin 1
NCBI Official Symbol:	LECT1
NCBI Official Synonym Symbols:	CHM1; CHM-I; BRICD3; MYETS1
NCBI Protein Information:	leukocyte cell-derived chemotaxin 1
UniProt Protein Name:	Leukocyte cell-derived chemotaxin 1
UniProt Synonym Protein Names:	Chondrosurfactant protein; CH-SP
Protein Family:	Leukocyte cell-derived chemotaxin
UniProt Gene Name:	LECT1
UniProt Entry Name:	LECT1_HUMAN

	Western blot analysis of extracts of mouse liver, using LECT1 antibody (CAB6644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using LECT1 antibody (CAB6644). Blue: DAPI for nuclear staining.