Anti-LDL Receptor (LDLR) Antibody (CAB20808)
- SKU:
- CAB20808
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Goat
- Isotype:
- IgG
Description
| Antibody Name: | Anti-LDL Receptor (LDLR) Antibody (CAB20808) |
| Antibody SKU: | CAB20808 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1). |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, HepG2, RAW264.7, Mouse liver, Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TVEI VTMS HQAL GDVA GRGN EKKP SSVR ALSI VLPI VLLV FLCL GVFL LWKN WRLK NINS INFD NPVY QKTT EDEV HICH NQDG YSYP SRQM VSLE DDVA |
| Cellular Location: | Cell membrane, Cell surface, Early endosome, Endomembrane system, Golgi apparatus, Late endosome, Lysosome, Membrane, Single-pass type I membrane protein, clathrin-coated pit |
| Calculated MW: | 75kDa/76kDa/82kDa/90kDa/95kDa |
| Observed MW: | 100-160KDa |
| Synonyms: | LDLR, FH, FHC, LDLCQ2 |
| Background: | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants. |
 | Immunofluorescence analysis of HepG2 cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of A-431 cells, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
 | Immunofluorescence analysis of HeLa cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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