KO Validated Antibodies 2

Anti-LDHB Antibody (CAB18096)[KO Validated]

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SKU:
CAB18096
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-LDHB Antibody [KO Validated]
Antibody SKU:CAB18096
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human LDHB (NP_002291.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human LDHB (NP_002291.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATL KEKL IAPV AEEE ATVP NNKI TVVG VGQV GMAC AISI LGKS LADE LALV DVLE DKLK GEMM DLQH GSLF LQTP KIVA DKDY SVTA NSKI VVVT AGVR QQEG ESRL NLVQ RNVN VFKF IIPQ IVKY SPDC IIIV VSNP VDIL TYVT WKLS GLPK HRVI GSGC NLDS ARFR YLMA EKLG IHPS SCHG WILG EHGD SSVA VWSG VNVA GVSL QELN PEMG TDND SENW KEVH KMVV ESAY EVIK LKGY TNWA IGLS VADL IESM LKNL SRIH PVST MVKG MYGI ENEV FLSL PCIL NARG LTSV INQK LKDD EVAQ LKKS ADTL WDIQ KDLK DL
Gene ID:3945
Uniprot:P07195
Cellular Location:Cytoplasm
Calculated MW:36kDa
Observed MW:37kDa
Anti-LDHB Antibody [KO Validated] (CAB18096)Western blot analysis of extracts from normal (control) and LDHB knockout (KO) 293T cells, using LDHB antibody (CAB18096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:LDHB, HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5
Background:This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
NCBI Summary:This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
UniProt Code:P07195
NCBI GenInfo Identifier:126041
NCBI Gene ID:3945
NCBI Accession:P07195.2
UniProt Related Accession:P07195
Molecular Weight:~ 37kDa
NCBI Full Name:L-lactate dehydrogenase B chain
NCBI Synonym Full Names:lactate dehydrogenase B
NCBI Official Symbol:LDHB  
NCBI Official Synonym Symbols:LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281  
NCBI Protein Information:L-lactate dehydrogenase B chain
UniProt Protein Name:L-lactate dehydrogenase B chain
UniProt Synonym Protein Names:LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:LDHB  
UniProt Entry Name:LDHB_HUMAN
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