Cell Biology Antibodies 2
Anti-LDHA Antibody (CAB1146)
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- SKU:
- CAB1146
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LDHA Antibody |
| Antibody SKU: | CAB1146 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, 293T, NIH/3T3, K-562, Jurkat, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATL KDQL IYNL LKEE QTPQ NKIT VVGV GAVG MACA ISIL MKDL ADEL ALVD VIED KLKG EMMD LQHG SLFL RTPK IVSG KDYN VTAN SKLV IITA GARQ QEGE SRLN LVQR NVNI FKFI IPNV VKYS PNCK LLIV SNPV DILT YVAW KISG FPKN RVIG SGCN LDSA RFRY LMGE RLGV HPLS CHGW VLGE HGDS SVPV WSGM NVAG VSLK TLHP DLGT DKDK EQWK EVHK QVVE SAYE VIKL KGYT SWAI GLSV ADLA ESIM KNLR RVHP VSTM IKGL YGIK DDVF LSVP CILG QNGI SDLV KVTL TSEE EARL KKSA DTLW GIQK ELQF |
| Gene ID: | 3939 |
| Uniprot: | P00338 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 26kDa/30kDa/36kDa/39kDa |
| Observed MW: | 37kDa |
| Synonyms: | GSD11, HEL-S-133P, LDHM, PIG19, LDHA |
| Background: | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. |
| NCBI Summary: | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P00338 |
| NCBI GenInfo Identifier: | 126047 |
| NCBI Gene ID: | 3939 |
| NCBI Accession: | P00338.2 |
| UniProt Secondary Accession: | P00338,Q53G53, Q6IBM7, Q6ZNV1, Q9UDE8, Q9UDE9, B4DKQ2 B7Z5E3, D3DQY3, F8W819, |
| UniProt Related Accession: | P00338 |
| Molecular Weight: | 26,712 Da |
| NCBI Full Name: | L-lactate dehydrogenase A chain |
| NCBI Synonym Full Names: | lactate dehydrogenase A |
| NCBI Official Symbol: | LDHA |
| NCBI Official Synonym Symbols: | LDHM; GSD11; PIG19; HEL-S-133P |
| NCBI Protein Information: | L-lactate dehydrogenase A chain |
| UniProt Protein Name: | L-lactate dehydrogenase A chain |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59 |
| Protein Family: | L-lactate dehydrogenase |
| UniProt Gene Name: | LDHA |
| UniProt Entry Name: | LDHA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LDHA antibody (CAB1146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of A549 cells using LDHA antibody (CAB1146). Blue: DAPI for nuclear staining. |
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