Antibody Name:	Anti-LDB3 Antibody
Antibody SKU:	CAB7462
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human LDB3 (NP_001073585.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, MCF7, U-937, Raji, BT-474, Mouse skeletal muscle, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human LDB3 (NP_001073585.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSYS VTLT GPGP WGFR LQGG KDFN MPLT ISRI TPGS KAAQ SQLS QGDL VVAI DGVN TDTM THLE AQNK IKSA SYNL SLTL QKSK RPIP ISTT APPV QTPL PVIP HQKV VVNS PANA DYQE RFNP SALK DSAL STHK PIEV KGLG GKAT IIHA QYNT PISM YSQD AIMD AIAG QAQA QGSD FSGS LPIK DLAV DSAS PVYQ AVIK SQNK PEDE ADEW ARRS SNLQ SRSF RILA QMTG TEFM QDPD EEAL RRSR ERFE TERN SPRF AKLR NWHH GLSA QILN VKS
Gene ID:	11155
Uniprot:	O75112
Cellular Location:	Cell projection, Cytoplasm, Z line, cytoskeleton, myofibril, perinuclear region, pseudopodium, sarcomere
Calculated MW:	30kDa/35kDa/42kDa/50kDa/66kDa/77kDa/78kDa
Observed MW:	50kDa/90kDa

Synonyms:

LDB3, CMD1C, CMH24, CMPD3, CYPHER, LDB3Z1, LDB3Z4, LVNC3, MFM4, ORACLE, PDLIM6, ZASP

Background:

This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.

UniProt Protein Function:	LDB3: May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton. Defects in LDB3 are the cause of cardiomyopathy dilated type 1C (CMD1C). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in LDB3 are the cause of left ventricular non- compaction type 3 (LVNC3). Left ventricular non- compaction is characterized by numerous prominent trabeculations and deep intertrabecular recesses in hypertrophied and hypokinetic segments of the left ventricle. Defects in LDB3 are the cause of myopathy myofibrillar type 4 (MFM4). A neuromuscular disorder characterized by distal and proximal muscle weakness with signs of cardiomyopathy and neuropathy. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 10q22.3-q23.2 Cellular Component: cytoskeleton; perinuclear region of cytoplasm; pseudopodium; Z disc Molecular Function:protein binding; protein kinase C binding; zinc ion binding; cytoskeletal protein binding; muscle alpha-actinin binding Biological Process: sarcomere organization Disease: Myopathy, Myofibrillar, 4
NCBI Summary:	This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]
UniProt Code:	O75112
NCBI GenInfo Identifier:	83288256
NCBI Gene ID:	11155
NCBI Accession:	O75112.2
UniProt Secondary Accession:	O75112,Q5K6N9, Q5K6P0, Q5K6P1, Q96FH2, Q9Y4Z3, Q9Y4Z4 Q9Y4Z5, A2TDB7, A6NIV4, B4E3K3,
UniProt Related Accession:	O75112
Molecular Weight:	727
NCBI Full Name:	LIM domain-binding protein 3
NCBI Synonym Full Names:	LIM domain binding 3
NCBI Official Symbol:	LDB3
NCBI Official Synonym Symbols:	MFM4; ZASP; CMD1C; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6
NCBI Protein Information:	LIM domain-binding protein 3; LDB3; protein cypher; PDZ and LIM domain 6; Z-band alternatively spliced PDZ motif protein; Z-band alternatively spliced PDZ-motif protein; cardiomyopathy, dilated 1C (autosomal dominant)
UniProt Protein Name:	LIM domain-binding protein 3
UniProt Synonym Protein Names:	Protein cypher; Z-band alternatively spliced PDZ-motif protein
Protein Family:	LIM domain-binding protein
UniProt Gene Name:	LDB3
UniProt Entry Name:	LDB3_HUMAN

	Western blot analysis of extracts of various cell lines, using LDB3 antibody (CAB7462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse stomach using LDB3 Rabbit pAb (CAB7462) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of human placenta cells using LDB3 antibody (CAB7462) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat heart cells using LDB3 antibody (CAB7462) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse heart cells using LDB3 antibody (CAB7462) at dilution of 1:100. Blue: DAPI for nuclear staining.