Autophagy Antibodies
Anti-LC3B Antibody (CAB5601)
- SKU:
- CAB5601
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-LC3B Antibody |
| Antibody SKU: | CAB5601 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, C6, Mouse heart, Mouse brain, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELI KIIR RRLQ LNAN QAFF LLVN GHSM VSVS TPIS EVYE |
| Gene ID: | 81631 |
| Uniprot: | Q9GZQ8 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton |
| Calculated MW: | 14kDa |
| Observed MW: | 14kDa/16kDa |
| Synonyms: | LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A/LC3B |
| Background: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| NCBI Summary: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9GZQ8 |
| NCBI GenInfo Identifier: | 17433141 |
| NCBI Gene ID: | 81631 |
| NCBI Accession: | Q9GZQ8.3 |
| UniProt Related Accession: | Q9GZQ8 |
| Molecular Weight: | |
| NCBI Full Name: | Microtubule-associated proteins 1A/1B light chain 3B |
| NCBI Synonym Full Names: | microtubule associated protein 1 light chain 3 beta |
| NCBI Official Symbol: | MAP1LC3B |
| NCBI Official Synonym Symbols: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| NCBI Protein Information: | microtubule-associated proteins 1A/1B light chain 3B |
| UniProt Protein Name: | Microtubule-associated proteins 1A/1B light chain 3B |
| UniProt Synonym Protein Names: | Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta |
| UniProt Gene Name: | MAP1LC3B |
| UniProt Entry Name: | MLP3B_HUMAN |
 | Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody (CAB5601) at 1:1000 dilution. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB5601) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using LC3B Rabbit pAb (CAB5601) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using LC3B Rabbit pAb (CAB5601) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using LC3B Rabbit pAb (CAB5601) at dilution of 1:100 (40x lens). |
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