Cell Cycle Antibodies 2
Anti-LATS1 Antibody (CAB17992)
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- SKU:
- CAB17992
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-LATS1 Antibody |
| Antibody SKU: | CAB17992 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human LATS1 |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | C2C12, SH-SY5Y |
| Immunogen: | Recombinant protein of human LATS1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 9113 |
| Uniprot: | O95835 |
| Cellular Location: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 76kDa/126kDa |
| Observed MW: | 130kDa |
 | Western blot - LATS1 Rabbit pAb (CAB17992) |
| Synonyms: | LATS1, WARTS, wts |
| Background: | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. |
| UniProt Protein Function: | LATS1: an AGC kinase of the NDR family of kinases. Localized to the mitotic apparatus and specifically phosphorylated during mitosis. A likely tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDC2 kinase activity, causing G2 arrest. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. Complexes with CDC2 in early mitosis. LATS1-associated CDC2 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated zyxin, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Knockout mice are susceptible to soft-tissue sarcomas and sensitive to chemical carcinogenesis. Human soft tissue sarcomas have downregulated, mutated, and/or hypermethylated LATS1. Transgenic expression blocks anchorage independent growth in culture and tumor growth in xenografts. Two alternatively spliced isoforms of the human proteinhave been described. |
| UniProt Protein Details: | Protein type:Protein kinase, AGC; Tumor suppressor; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; NDR family Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: cytosol; microtubule organizing center; spindle pole Molecular Function:ATP binding; magnesium ion binding; protein binding; protein kinase binding; protein serine/threonine kinase activity Biological Process: cell division; cytoplasmic sequestering of protein; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; hormone-mediated signaling; inner cell mass cell fate commitment; inner cell mass cellular morphogenesis; keratinocyte differentiation; mitosis; negative regulation of cyclin-dependent protein kinase activity; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of actin filament polymerization; regulation of organ growth; regulation of protein complex assembly; sister chromatid segregation |
| NCBI Summary: | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | O95835 |
| NCBI GenInfo Identifier: | 52783106 |
| NCBI Gene ID: | 9113 |
| NCBI Accession: | O95835.1 |
| UniProt Secondary Accession: | O95835,Q6PKD0, |
| UniProt Related Accession: | O95835 |
| Molecular Weight: | |
| NCBI Full Name: | Serine/threonine-protein kinase LATS1 |
| NCBI Synonym Full Names: | large tumor suppressor kinase 1 |
| NCBI Official Symbol: | LATS1 |
| NCBI Official Synonym Symbols: | wts; WARTS |
| NCBI Protein Information: | serine/threonine-protein kinase LATS1 |
| UniProt Protein Name: | Serine/threonine-protein kinase LATS1 |
| UniProt Synonym Protein Names: | Large tumor suppressor homolog 1; WARTS protein kinase; h-warts |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | LATS1 |
| UniProt Entry Name: | LATS1_HUMAN |
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