Antibody Name:	Anti-LASP1 Antibody
Antibody SKU:	CAB3941
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-205 of human LASP1 (NP_006139.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:3000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, BT-474, SKOV3, U-87MG, LO2, Mouse liver, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-205 of human LASP1 (NP_006139.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RMGP SGGE GMEP ERRD SQDG SSYR RPLE QQQP HHIP TSAP VYQQ PQQQ PVAQ SYGG YKEP AAPV SIQR SAPG GGGK
Gene ID:	3927
Uniprot:	Q14847
Cellular Location:	Cytoplasm, cell cortex, cytoskeleton
Calculated MW:	23kDa/29kDa/36kDa
Observed MW:	37kDa

Synonyms:

LASP1, Lasp-1, MLN50

Background:

This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer.

UniProt Protein Function:	Lasp-1: a member of the LIM protein subfamily, characterized by a LIM motif and SH3 domain. Functions as an actin-binding protein, possibly in cytoskeletal organization.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 17q11-q21.3 Cellular Component: cortical actin cytoskeleton; focal adhesion Molecular Function:actin filament binding; ion transmembrane transporter activity; protein binding; SH3/SH2 adaptor activity; zinc ion binding Biological Process: ion transport; positive regulation of signal transduction
NCBI Summary:	This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]
UniProt Code:	Q14847
NCBI GenInfo Identifier:	3122342
NCBI Gene ID:	3927
NCBI Accession:	Q14847.2
UniProt Secondary Accession:	Q14847,Q96ED2, Q96IG0, B4DGQ0,
UniProt Related Accession:	Q14847
Molecular Weight:	23,181 Da
NCBI Full Name:	LIM and SH3 domain protein 1
NCBI Synonym Full Names:	LIM and SH3 protein 1
NCBI Official Symbol:	LASP1
NCBI Official Synonym Symbols:	MLN50; Lasp-1
NCBI Protein Information:	LIM and SH3 domain protein 1
UniProt Protein Name:	LIM and SH3 domain protein 1
UniProt Synonym Protein Names:	Metastatic lymph node gene 50 protein; MLN 50
Protein Family:	LIM and SH3 domain protein
UniProt Gene Name:	LASP1
UniProt Entry Name:	LASP1_HUMAN

	Western blot analysis of extracts of various cell lines, using LASP1 antibody (CAB3941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using LASP1 Rabbit pAb (CAB3941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using LASP1 Rabbit pAb (CAB3941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using LASP1 Rabbit pAb (CAB3941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.