Antibody Name:	Anti-LARS Antibody
Antibody SKU:	CAB14890
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LARS (NP_064502.9).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	K-562, HeLa, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LARS (NP_064502.9).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAER KGTA KVDF LKKI EKEI QQKW DTER VFEV NASN LEKQ TSKG KYFV TFPY PYMN GRLH LGHT FSLS KCEF AVGY QRLK GKCC LFPF GLHC TGMP IKAC ADKL KREI ELYG CPPD FPDE EEEE EETS VKTE DIII KDKA KGKK SKAA AKAG SSKY QWGI MKSL GLSD EEIV KFSE AEHW LDYF PPLA IQDL KRMG LKVD WRRS FITT DVNP YYDS FVRW QFLT LRER NKIK FGKR YTIY SPKD GQPC MDHD RQTG EGVG PQEY TLLK LK
Gene ID:	51520
Uniprot:	Q9P2J5
Cellular Location:	Cytoplasm
Calculated MW:	55kDa/128kDa/134kDa
Observed MW:	134kDa

Synonyms:

LARS, HSPC192, ILFS1, LARS1, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, hr025Cl

Background:

This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed.

UniProt Protein Function:	LARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs. Belongs to the class-I aminoacyl-tRNA synthetase family.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Ligase; Translation; EC 6.1.1.4 Chromosomal Location of Human Ortholog: 5q32 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:leucine-tRNA ligase activity; protein binding Biological Process: leucyl-tRNA aminoacylation; tRNA aminoacylation for protein translation Disease: Infantile Liver Failure Syndrome 1
NCBI Summary:	This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015]
UniProt Code:	Q9P2J5
NCBI GenInfo Identifier:	48428689
NCBI Gene ID:	51520
NCBI Accession:	Q9P2J5.2
UniProt Secondary Accession:	Q9P2J5,Q2TU79, Q9NSE1, A2RRR4, A7E266, B4DJ10,
UniProt Related Accession:	Q9P2J5
Molecular Weight:	55,295 Da
NCBI Full Name:	Leucine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:	leucyl-tRNA synthetase
NCBI Official Symbol:	LARS
NCBI Official Synonym Symbols:	LRS; LEUS; LFIS; ILFS1; LARS1; LEURS; PIG44; RNTLS; HSPC192; hr025Cl
NCBI Protein Information:	leucine--tRNA ligase, cytoplasmic
UniProt Protein Name:	Leucine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:	Leucyl-tRNA synthetase; LeuRS
Protein Family:	Leucine--tRNA ligase
UniProt Gene Name:	LARS
UniProt Entry Name:	SYLC_HUMAN

	Western blot analysis of extracts of various cell lines, using LARS antibody (CAB14890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of L929 cells using LARS Rabbit pAb (CAB14890) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using LARS Rabbit pAb (CAB14890) at dilution of 1:100. Blue: DAPI for nuclear staining.