Cell Biology Antibodies 10
Anti-LAP3 Antibody (CAB7101)
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- SKU:
- CAB7101
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LAP3 Antibody |
| Antibody SKU: | CAB7101 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-519 of human LAP3 (NP_056991.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-519 of human LAP3 (NP_056991.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VFLE IHYK GSPN ANEP PLVF VGKG ITFD SGGI SIKA SANM DLMR ADMG GAAT ICSA IVSA AKLN LPIN IIGL APLC ENMP SGKA NKPG DVVR AKNG KTIQ VDNT DAEG RLIL ADAL CYAH TFNP KVIL NAAT LTGA MDVA LGSG ATGV FTNS SWLW NKLF EASI ETGD RVWR MPLF EHYT RQVV DCQL ADVN NIGK YRSA GACT AAAF LKEF VTHP KWAH LDIA GVMT NKDE VPYL RKGM TGRP TRTL IEFL LRFS QDNA |
| Gene ID: | 51056 |
| Uniprot: | P28838 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 52kDa/56kDa |
| Observed MW: | 56kDa |
| Synonyms: | LAP3, HEL-S-106, LAP, LAPEP, PEPS |
| Background: |
| UniProt Protein Function: | LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - glutathione; Protease; EC 3.4.11.5; Mitochondrial; EC 3.4.11.1; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 4p15.32 Cellular Component: cytoplasm; focal adhesion; nucleoplasm; nucleus |
| UniProt Code: | P28838 |
| NCBI GenInfo Identifier: | 124028615 |
| NCBI Gene ID: | 51056 |
| NCBI Accession: | P28838.3 |
| UniProt Secondary Accession: | P28838,Q6IAM6, Q6P0L6, Q9UQE3, B3KMQ3, |
| UniProt Related Accession: | P28838 |
| Molecular Weight: | 52,771 Da |
| NCBI Full Name: | Cytosol aminopeptidase |
| NCBI Synonym Full Names: | leucine aminopeptidase 3 |
| NCBI Official Symbol: | LAP3 |
| NCBI Official Synonym Symbols: | LAP; PEPS; LAPEP; HEL-S-106 |
| NCBI Protein Information: | cytosol aminopeptidase |
| UniProt Protein Name: | Cytosol aminopeptidase |
| UniProt Synonym Protein Names: | Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase (EC:3.4.11.5); Prolyl aminopeptidase |
| Protein Family: | Leucine aminopeptidase |
| UniProt Gene Name: | LAP3 |
| UniProt Entry Name: | AMPL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LAP3 antibody (CAB7101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using LAP3 antibody (CAB7101) at dilution of 1:100 (40x lens). |
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