Name: Anti-LAMTOR1 Antibody (CAB11619)
Brand: Cell Biology Antibodies 2
SKU: CAB11619
Availability: OutOfStock

Antibody Name:	Anti-LAMTOR1 Antibody
Antibody SKU:	CAB11619
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	B-cell, HeLa, Jurkat, U-87MG, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGCC YSSE NEDS DQDR EERK LLLD PSSP PTKA LNGA EPNY HSLP SART DEQA LLSS ILAK TASN IIDV SAAD SQGM EQHE YMDR ARQY STRL AVLS SSLT HWKK LPPL PSLT SQPH QVLA SEPI PFSD LQQV SRIA AYAY SALS QIRV DAKE ELVV QFGI P
Gene ID:	55004
Uniprot:	Q6IAA8
Cellular Location:	Cell membrane, Cytoplasmic side, Late endosome membrane, Lipid-anchor, Lysosome membrane
Calculated MW:	17kDa
Observed MW:	18kDa

Synonyms:	LAMTOR1, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho
Background:

UniProt Protein Function:	LAMTOR1: Regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids. Directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. Part of the Ragulator complex composed of LAMTOR1, LAMTOR2 and LAMTOR3. Interacts with LAMTOR2 and LAMTOR3; the interaction is direct. The Ragulator complex interacts with both the mTORC1 complex and heterodimers of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD. Interacts with RRAGB and RRAGD; the interaction is direct indicating that it probably constitutes the main RAG-interacting subunit of the Ragulator complex. Interacts with MMP14. Interacts with CDKN1B; prevents the interaction of CDKN1B with RHOA leaving RHOA in a form accessible to activation by ARHGEF2.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 11q13.4 Cellular Component: late endosome membrane; lipid raft; lysosomal membrane; lysosome; plasma membrane Molecular Function:GTPase binding; guanyl-nucleotide exchange factor activity; protein binding; protein complex scaffold Biological Process: cell cycle arrest; cell growth; cholesterol homeostasis; endosome localization; endosome organization and biogenesis; lysosome localization; lysosome organization and biogenesis; macroautophagy; neutrophil degranulation; positive regulation of MAPKKK cascade; positive regulation of TOR signaling pathway; regulation of receptor recycling
UniProt Code:	Q6IAA8
NCBI GenInfo Identifier:	125863645
NCBI Gene ID:	55004
NCBI Accession:	Q6IAA8.2
UniProt Secondary Accession:	Q6IAA8,Q8WZ09, Q9NWT0,
UniProt Related Accession:	Q6IAA8
Molecular Weight:	18kDa
NCBI Full Name:	Ragulator complex protein LAMTOR1
NCBI Synonym Full Names:	late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
NCBI Official Symbol:	LAMTOR1
NCBI Official Synonym Symbols:	p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
NCBI Protein Information:	ragulator complex protein LAMTOR1
UniProt Protein Name:	Ragulator complex protein LAMTOR1
UniProt Synonym Protein Names:	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; Lipid raft adaptor protein p18; Protein associated with DRMs and endosomes; p27Kip1-releasing factor from RhoA; p27RF-Rho
Protein Family:	Ragulator complex protein
UniProt Gene Name:	LAMTOR1

	Western blot analysis of extracts of various cell lines, using LAMTOR1 antibody (CAB11619) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using LAMTOR1 Polyclonal Antibody (CAB11619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using LAMTOR1 Polyclonal Antibody (CAB11619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using LAMTOR1 Polyclonal Antibody (CAB11619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.