Cell Biology Antibodies 6
Anti-LAMC1 Antibody (CAB16020)
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- SKU:
- CAB16020
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LAMC1 Antibody |
| Antibody SKU: | CAB16020 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1300-1609 of human LAMC1 (NP_002284.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, Mouse heart, Mouse kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1300-1609 of human LAMC1 (NP_002284.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IDQK LKDY EDLR EDMR GKEL EVKN LLEK GKTE QQTA DQLL ARAD AAKA LAEE AAKK GRDT LQEA NDIL NNLK DFDR RVND NKTA AEEA LRKI PAIN QTIT EANE KTRE AQQA LGSA AADA TEAK NKAH EAER IASA VQKN ATST KAEA ERTF AEVT DLDN EVNN MLKQ LQEA EKEL KRKQ DDAD QDMM MAGM ASQA AQEA EINA RKAK NSVT SLLS IIND LLEQ LGQL DTVD LNKL NEIE GTLN KAKD EMKV SDLD RKVS DLEN EAKK QEAA IMDY NRDI EEIM KDIR NLED IRKT LPSG CFNT PSIE KP |
| Gene ID: | 3915 |
| Uniprot: | P11047 |
| Cellular Location: | |
| Calculated MW: | 177kDa |
| Observed MW: | 240kDa |
| Synonyms: | LAMC1, LAMB2 |
| Background: | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. |
| UniProt Protein Function: | LAMC1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q31 Cellular Component: extracellular matrix; extracellular space; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane Molecular Function:extracellular matrix structural constituent; glycosphingolipid binding Biological Process: axon guidance; extracellular matrix disassembly; cell migration; extracellular matrix organization and biogenesis; hemidesmosome assembly; protein complex assembly; cell adhesion; positive regulation of epithelial cell proliferation; endoderm development Disease: Pelvic Organ Prolapse, Susceptibility To |
| NCBI Summary: | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P11047 |
| NCBI GenInfo Identifier: | 224471885 |
| NCBI Gene ID: | 3915 |
| NCBI Accession: | P11047.3 |
| UniProt Secondary Accession: | P11047,Q5VYE7, |
| UniProt Related Accession: | P11047 |
| Molecular Weight: | 177,603 Da |
| NCBI Full Name: | Laminin subunit gamma-1 |
| NCBI Synonym Full Names: | laminin, gamma 1 (formerly LAMB2) |
| NCBI Official Symbol: | LAMC1 |
| NCBI Official Synonym Symbols: | LAMB2 |
| NCBI Protein Information: | laminin subunit gamma-1; S-LAM gamma; laminin B2 chain; S-laminin subunit gamma; laminin-2 subunit gamma; laminin-3 subunit gamma; laminin-4 subunit gamma; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit gamma; laminin-9 subunit gamma; laminin-10 subunit gamma; laminin-11 subunit gamma |
| UniProt Protein Name: | Laminin subunit gamma-1 |
| UniProt Synonym Protein Names: | Laminin B2 chain; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; S-laminin subunit gamma; S-LAM gamma |
| Protein Family: | Laminin |
| UniProt Gene Name: | LAMC1 |
| UniProt Entry Name: | LAMC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LAMC1 antibody (CAB16020) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using LAMC1 antibody (CAB16020) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human uterus using LAMC1 antibody (CAB16020) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human uterine cancer using LAMC1 antibody (CAB16020) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat brain using LAMC1 antibody (CAB16020) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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