Cell Biology Antibodies 5
Anti-LAMA4 Antibody (CAB15286)
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- SKU:
- CAB15286
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-LAMA4 Antibody |
| Antibody SKU: | CAB15286 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LAMA4 (NP_001098678.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LAMA4 (NP_001098678.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MALS SAWR SVLP LWLL WSAA CSRA ASGD DNAF PFDI EGSS AVGR QDPP ETSE PRVA LGRL PPAA EVQC PCHC HPAG APAP PRAV PHSS FSLS PPLS SPQC |
| Gene ID: | 3910 |
| Uniprot: | Q16363 |
| Cellular Location: | Secreted, basement membrane, extracellular matrix, extracellular space |
| Calculated MW: | 12kDa/201kDa/202kDa |
| Observed MW: | 203kDa |
| Synonyms: | LAMA4, CMD1JJ, LAMA3, LAMA4*-1 |
| Background: | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1, 200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | LAMA4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Extracellular matrix Chromosomal Location of Human Ortholog: 6q21 Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region Molecular Function:extracellular matrix structural constituent; protein binding Biological Process: extracellular matrix organization and biogenesis Disease: Cardiomyopathy, Dilated, 1jj |
| NCBI Summary: | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q16363 |
| NCBI GenInfo Identifier: | 292495093 |
| NCBI Gene ID: | 3910 |
| NCBI Accession: | Q16363.4 |
| UniProt Secondary Accession: | Q16363,Q14731, Q14735, Q15335, Q4LE44, Q5SZG8, Q9BTB8 Q9UE18, Q9UJN9, |
| UniProt Related Accession: | Q16363 |
| Molecular Weight: | 12,819 Da |
| NCBI Full Name: | Laminin subunit alpha-4 |
| NCBI Synonym Full Names: | laminin subunit alpha 4 |
| NCBI Official Symbol: | LAMA4 |
| NCBI Official Synonym Symbols: | LAMA3; CMD1JJ; LAMA4*-1 |
| NCBI Protein Information: | laminin subunit alpha-4 |
| UniProt Protein Name: | Laminin subunit alpha-4 |
| UniProt Synonym Protein Names: | Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha |
| Protein Family: | Laminin |
| UniProt Gene Name: | LAMA4 |
| UniProt Entry Name: | LAMA4_HUMAN |
 | Western blot analysis of extracts of 293T cells, using LAMA4 antibody (CAB15286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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