Anti-lacZ Antibody (MACO0616)
- SKU:
- MACO0616
- Product Type:
- Antibody
- Reactivity:
- Bacteria - Escherichia coli
- Host Species:
- Mouse
- Isotype:
- IgG1
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Applications:
- IP
- Applications:
- FC
- Antibody Type:
- Monoclonal Antibody
- Conjugation:
- Unconjugated
Description
| Product Name: | Anti-lacZ Antibody |
| Product Sku: | MACO0616 |
| Size: | 50ul |
| Host Species: | Mouse |
| Tested Applications: | ELISA, WB, IF, IP, FC |
| Recommended Dilutions: | WB:1:1000-1:64000, IF:1:50-1:200, IP:1ul-4ul, FC:1:100-1:300 |
| Species Reactivity: | Escherichia coli |
| Immunogen: | Recombinant Escherichia coli Beta-galactosidase protein |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein A purified |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Conjugate: | Non-conjugated |
| Background: | Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. |
| Synonyms: | Beta-galactosidase (Beta-gal) (Lactase), lacZ |
 | Immunofluorescence staining of 293T cells transfected with MACO0616 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody |
 | Immunoprecipitating lacZ in 293T transfected whole cell lysate Lane 1: Mouse control IgG instead of MACO0616 in 293T transfected whole cell lysate. Lane 2: MACO0616 (2µl) + 293T transfected whole cell lysate (500µg) Lane 3: 293T transfected whole cell lysate ( 20µg) For western blotting, the blot was detected with MACO0616 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 |
 | Western Blot Positive WB detected in Recombinant protein ( 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg) All lanes: lacZ antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 117 kDa Observed band size: 130 kDa |
| UniProt Protein Function: | lacZ: Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527] Protein type: EC 3.2.1.23 |
| UniProt Protein Details: | |
| NCBI Summary: | Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527]. |
| UniProt Code: | P00722 |
| NCBI GenInfo Identifier: | 114939 |
| NCBI Gene ID: | 945006 |
| NCBI Accession: | P00722.2 |
| UniProt Secondary Accession: | P00722,Q2MC80 |
| UniProt Related Accession: | P00722 |
| Molecular Weight: | |
| NCBI Full Name: | Beta-galactosidase |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | lacZ |
| NCBI Official Synonym Symbols: | ECK0341; JW0335 |
| NCBI Protein Information: | beta-D-galactosidase |
| UniProt Protein Name: | Beta-galactosidase |
| UniProt Synonym Protein Names: | Lactase |
| Protein Family: | Beta-galactosidase |
| UniProt Gene Name: | lacZ |
| UniProt Entry Name: | BGAL_ECOLI |
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