Description
Antibody Name: | Anti-Lactate Dehydrogenase C Antibody (CAB20866) |
Antibody SKU: | CAB20866 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human Lactate Dehydrogenase C. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human |
Positive Samples: | 293T, U-937 |
Immunogen: | Recombinant protein of Human Lactate Dehydrogenase C. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm |
Calculated MW: | 36kDa |
Observed MW: | 36KDa |
Synonyms: | LDHC, CT32, LDH3, LDHX |
Background: | Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. |
Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase C antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |