Name: Anti-KYNU Antibody (CAB6643)
Brand: Metabolism Antibodies 2
SKU: CAB6643
Availability: OutOfStock

Antibody Name:	Anti-KYNU Antibody
Antibody SKU:	CAB6643
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human KYNU (NP_001028170.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, THP-1, NCI-H460, HepG2, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human KYNU (NP_001028170.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPS SLEL PADT VQRI AAEL KCHP TDER VALH LDEE DKLR HFRE CFYI PKIQ DLPP VDLS LVNK DENA IYFL GNSL GLQP KMVK TYLE EELD KWAK IAAY GHEV GKRP WITG DESI VGLM KDIV GANE KEIA LMNA LTVN LHLL MLSF FKPT PKRY KILL EAKA FPSD HYAI ESQL QLHG LNIE ESMR MIKP REGE ETLR IEDI LEVI EKEG DSIA VILF SGVH FYTG QHFN IPAI TKAG QAKG CYVG FDLA HAVG NVEL YLHD WGVD FACW CSYK YLNA GAGG IAGA FIHE KHAH TIKP ARSE FFN
Gene ID:	8942
Uniprot:	Q16719
Cellular Location:	Cytoplasm
Calculated MW:	34kDa/52kDa
Observed MW:	52kDa

Synonyms:	KYNU, KYNUU
Background:	Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.7.1.3; Mitochondrial; Amino Acid Metabolism - tryptophan; Hydrolase Chromosomal Location of Human Ortholog: 2q22.2 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol Molecular Function:protein homodimerization activity; kynureninase activity; pyridoxal phosphate binding Biological Process: anthranilate metabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; quinolinate biosynthetic process; tryptophan catabolic process Disease: Hydroxykynureninuria
NCBI Summary:	Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
UniProt Code:	Q16719
NCBI GenInfo Identifier:	3913982
NCBI Gene ID:	8942
NCBI Accession:	Q16719.1
UniProt Secondary Accession:	Q16719,Q6I9T2, Q9BVW3, B2RCZ5, D3DP79,
UniProt Related Accession:	Q16719
Molecular Weight:	34,635 Da
NCBI Full Name:	Kynureninase
NCBI Synonym Full Names:	kynureninase
NCBI Official Symbol:	KYNU
NCBI Protein Information:	kynureninase; KYNU; L-kynurenine hydrolase
UniProt Protein Name:	Kynureninase
UniProt Synonym Protein Names:	L-kynurenine hydrolase
UniProt Gene Name:	KYNU
UniProt Entry Name:	KYNU_HUMAN

	Western blot analysis of extracts of various cell lines, using KYNU antibody (CAB6643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse heart using KYNU antibody (CAB6643) at dilution of 1:100 (40x lens).