Cell Biology Antibodies 5
Anti-KSR1 Antibody (CAB15118)
- SKU:
- CAB15118
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-KSR1 Antibody |
| Antibody SKU: | CAB15118 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-420 of human KSR1 (NP_055053.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HeLa, Jurkat, mouse brain, mouse spleen, mouse testis, mouse kidney, mouse lung, rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-420 of human KSR1 (NP_055053.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SSPA PFPT SSNP SSAT TPPN PSPG QRDS RFNF PAAY FIHH RQQF IFPV PSAG HCWK CLLI AESL KENA FNIS AFAH AAPL PEAA DGTR LDDQ PKAD VLEA H |
| Gene ID: | 8844 |
| Uniprot: | Q8IVT5 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum membrane, Membrane, Peripheral membrane protein |
| Calculated MW: | 84kDa/87kDa/97kDa/102kDa |
| Observed MW: | 110kDa |
| Synonyms: | KSR1, KSR, RSU2 |
| Background: |
| UniProt Protein Function: | KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RAF family Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; protein complex Molecular Function:ATP binding; MAP-kinase scaffold activity; metal ion binding; mitogen-activated protein kinase kinase binding; protein binding; protein C-terminus binding; protein kinase activity Biological Process: activation of MAPKK activity; axon guidance; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway |
| UniProt Code: | Q8IVT5 |
| NCBI GenInfo Identifier: | 677286751 |
| NCBI Gene ID: | 8844 |
| NCBI Accession: | Q8IVT5.3 |
| UniProt Secondary Accession: | Q8IVT5,Q13476, F8WEA9, H7BYU0, |
| UniProt Related Accession: | Q8IVT5 |
| Molecular Weight: | 84,697 Da |
| NCBI Full Name: | Kinase suppressor of Ras 1 |
| NCBI Synonym Full Names: | kinase suppressor of ras 1 |
| NCBI Official Symbol: | KSR1 |
| NCBI Official Synonym Symbols: | KSR; RSU2 |
| NCBI Protein Information: | kinase suppressor of Ras 1 |
| UniProt Protein Name: | Kinase suppressor of Ras 1 |
| Protein Family: | Kinase suppressor of Ras |
| UniProt Gene Name: | KSR1 |
| UniProt Entry Name: | KSR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KSR1 antibody (CAB15118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using KSR1 Polyclonal Antibody (CAB15118) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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