Antibody Name:	Anti-KRT81 Antibody
Antibody SKU:	CAB3940
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 240-390 of human KRT81 (NP_002272.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Raji, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 240-390 of human KRT81 (NP_002272.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RRLY EEEI LILQ SHIS DTSV VVKL DNSR DLNM DCII AEIK AQYD DIVT RSRA EAES WYRS KCEE MKAT VIRH GETL RRTK EEIN ELNR MIQR LTAE VENA KCQN SKLE AAVA QSEQ QGEA ALSD ARCK LAEL EGAL QKAK QDMA CLIR EYQ
Gene ID:	3887
Uniprot:	Q14533
Cellular Location:
Calculated MW:	54kDa
Observed MW:	55kDa

Synonyms:

KRT81, HB1, Hb-1, KRTHB1, MLN137, ghHkb1, hHAKB2-1

Background:

The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.

UniProt Protein Function:	K81: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 12q13 Cellular Component: extracellular space; keratin filament Molecular Function:protein binding; structural molecule activity Disease: Monilethrix
NCBI Summary:	The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14533
NCBI GenInfo Identifier:	311033435
NCBI Gene ID:	3887
NCBI Accession:	Q14533.3
UniProt Secondary Accession:	Q14533,Q14846, Q16274, Q17R48, Q8WU52, Q9BR74,
UniProt Related Accession:	Q14533
Molecular Weight:	505
NCBI Full Name:	Keratin, type II cuticular Hb1
NCBI Synonym Full Names:	keratin 81
NCBI Official Symbol:	KRT81
NCBI Official Synonym Symbols:	HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1
NCBI Protein Information:	keratin, type II cuticular Hb1; K81; ghHb1; MLN 137; keratin-81; hair keratin K2.9; type-II keratin Kb21; keratin, hair, basic, 1; hard keratin, type II, 1; type II hair keratin Hb1; metastatic lymph node 137 gene protein
UniProt Protein Name:	Keratin, type II cuticular Hb1
UniProt Synonym Protein Names:	Hair keratin K2.9; Keratin, hair, basic, 1; Keratin-81; K81; Metastatic lymph node 137 gene protein; MLN 137; Type II hair keratin Hb1; Type-II keratin Kb21; ghHKb1; ghHb1
Protein Family:	Keratin
UniProt Gene Name:	KRT81
UniProt Entry Name:	KRT81_HUMAN

	Western blot analysis of extracts of various cell lines, using KRT81 antibody (CAB3940) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using KRT81 antibody (CAB3940) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human mammary cancer using KRT81 antibody (CAB3940) at dilution of 1:100 (40x lens).