Antibody Name:	Anti-KRT6B Antibody
Antibody SKU:	CAB7696
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human KRT6B (NP_005546.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human KRT6B (NP_005546.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAST STTI RSHS SSRR GFSA NSAR LPGV SRSG FSSI SVSR SRGS GGLG GACG GAGF GSRS LYGL GGSK RISI GGGS CAIS GGYG SRAG GSYG FGGA GSGF GFGG GAGI GFGL GGGA GLAG GFGG PGFP VCPP GGIQ EVTV NQSL LTPL NLQI DPAI QRVR AEER EQIK TLNN KFAS FIDK VRFL EQQN KVLD TKWT LLQE QGTK TVRQ NLEP LFEQ YINN LRRQ LDNI VGER GRLD SELR NMQD LVED LKNK YEDE INKR TAAE NEFV TLKK DVDA AYMN
Gene ID:	3854
Uniprot:	P04259
Cellular Location:
Calculated MW:	60kDa
Observed MW:	60kDa

Synonyms:

KRT6B, CK-6B, CK6B, K6B, KRTL1, PC2, PC4

Background:

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

UniProt Protein Function:	KRT6B: Defects in KRT6B are a cause of pachyonychia congenita type 2 (PC2); also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth. Belongs to the intermediate filament family.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 12q13.13 Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: ectoderm development Disease: Pachyonychia Congenita 4
NCBI Summary:	The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
UniProt Code:	P04259
NCBI GenInfo Identifier:	238054404
NCBI Gene ID:	3854
NCBI Accession:	P04259.5
UniProt Secondary Accession:	P04259,P48669,
UniProt Related Accession:	P04259
Molecular Weight:	60,067 Da
NCBI Full Name:	Keratin, type II cytoskeletal 6B
NCBI Synonym Full Names:	keratin 6B
NCBI Official Symbol:	KRT6B
NCBI Official Synonym Symbols:	K6B; PC2; PC4; CK6B; CK-6B; KRTL1
NCBI Protein Information:	keratin, type II cytoskeletal 6B
UniProt Protein Name:	Keratin, type II cytoskeletal 6B
UniProt Synonym Protein Names:	Cytokeratin-6B; CK-6B; Keratin-6B; K6B; Type-II keratin Kb10
Protein Family:	Keratin
UniProt Gene Name:	KRT6B
UniProt Entry Name:	K2C6B_HUMAN

	Western blot analysis of extracts of mouse eye, using KRT6B antibody (CAB7696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using KRT6B antibody (CAB7696) at dilution of 1:100. Blue: DAPI for nuclear staining.