Cell Biology Antibodies 8

Anti-KRT5 Antibody (CAB2662)

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SKU:
CAB2662
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KRT5 Antibody
Antibody SKU:CAB2662
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human KRT5
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-431, Mouse kidney, Mouse thymus, Mouse skeletal muscle
Immunogen:Recombinant protein of human KRT5
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3852
Uniprot:P13647
Cellular Location:
Calculated MW:62kDa
Observed MW:70kDa
Synonyms:KRT5, CK5, DDD, DDD1, EBS2, K5, KRT5A, keratin 5
Background:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
UniProt Protein Function:K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: mitochondrion; membrane; cytoplasm; keratin filament; plasma membrane; intermediate filament; cytosol; nucleus

Molecular Function:protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; hemidesmosome assembly

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type

NCBI Summary:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
UniProt Code:P13647
NCBI GenInfo Identifier:143811411
NCBI Gene ID:3852
NCBI Accession:P13647.3
UniProt Secondary Accession:P13647,Q6PI71, Q6UBJ0, Q8TA91,
UniProt Related Accession:P13647
Molecular Weight:590
NCBI Full Name:Keratin, type II cytoskeletal 5
NCBI Synonym Full Names:keratin 5
NCBI Official Symbol:KRT5  
NCBI Official Synonym Symbols:K5; CK5; DDD; DDD1; EBS2; KRT5A  
NCBI Protein Information:keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)
UniProt Protein Name:Keratin, type II cytoskeletal 5
UniProt Synonym Protein Names:58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Protein Family:Keratin
UniProt Gene Name:KRT5  
UniProt Entry Name:K2C5_HUMAN
Anti-KRT5 Antibody (CAB2662)Western blot analysis of extracts of various cell lines, using KRT5 antibody (CAB2662) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-KRT5 Antibody (CAB2662)Immunohistochemistry of paraffin-embedded Human esophageal cancer using KRT5 Rabbit pAb (CAB2662) at dilution of 1:100 (40x lens).
Anti-KRT5 Antibody (CAB2662)Immunofluorescence analysis of L929 cells using KRT5 Rabbit pAb (CAB2662) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-KRT5 Antibody (CAB2662)Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug KRT5 antibody (CAB2662). Western blot was performed from the immunoprecipitate using KRT5 antibody (CAB2662) at a dilition of 1:5000.
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