Cell Biology Antibodies 4
Anti-KRT36 Antibody (CAB13759)
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- SKU:
- CAB13759
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-KRT36 Antibody |
| Antibody SKU: | CAB13759 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 388-467 of human KRT36 (NP_003762.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | HeLa, LO2, BxPC-3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 388-467 of human KRT36 (NP_003762.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GEIA TYRH LLEG EDCK LPPQ PCAT ACKP VIRV PSVP PVPC VPSV PCTP APQV GTQI RTIT EEIR DGKV ISSR EHVQ SRPL |
| Gene ID: | 8689 |
| Uniprot: | O76013 |
| Cellular Location: | |
| Calculated MW: | 47kDa/52kDa |
| Observed MW: | 52kDa |
| Synonyms: | KRT36, HA6, KRTHA6, hHa6 |
| Background: | The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. |
| UniProt Protein Function: | K36: a type I hair keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of hair/microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2 |
| NCBI Summary: | The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O76013 |
| NCBI GenInfo Identifier: | 6685565 |
| NCBI Gene ID: | 8689 |
| NCBI Accession: | O76013.1 |
| UniProt Secondary Accession: | O76013,Q86XG4, |
| UniProt Related Accession: | O76013 |
| Molecular Weight: | 47,485 Da |
| NCBI Full Name: | Keratin, type I cuticular Ha6 |
| NCBI Synonym Full Names: | keratin 36 |
| NCBI Official Symbol: | KRT36 |
| NCBI Official Synonym Symbols: | HA6; hHa6; KRTHA6 |
| NCBI Protein Information: | keratin, type I cuticular Ha6 |
| UniProt Protein Name: | Keratin, type I cuticular Ha6 |
| UniProt Synonym Protein Names: | Hair keratin, type I Ha6; Keratin-36; K36 |
| Protein Family: | Keratin |
| UniProt Gene Name: | KRT36 |
| UniProt Entry Name: | KRT36_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KRT36 antibody (CAB13759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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