Immunology Antibodies 1

Anti-KRT19 Antibody (CAB0247)

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SKU:
CAB0247
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-KRT19 Antibody
Antibody SKU:CAB0247
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 241-400 of human KRT19 (NP_002267.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse stomach
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 241-400 of human KRT19 (NP_002267.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PGTD LAKI LSDM RSQY EVMA EQNR KDAE AWFT SRTE ELNR EVAG HTEQ LQMS RSEV TDLR RTLQ GLEI ELQS QLSM KAAL EDTL AETE ARFG AQLA HIQA LISG IEAQ LGDV RADS ERQN QEYQ RLMD IKSR LEQE IATY RSLL EGQE DHYN NLSA SKVL
Gene ID:3880
Uniprot:P08727
Cellular Location:
Calculated MW:44kDa
Observed MW:46kDa
Synonyms:CK19, K19, K1CS, KRT19
Background:The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
UniProt Protein Function:Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Ref.14
UniProt Protein Details:

Subunit structure: Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein. Ref.13 Ref.14

Tissue specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. Ref.2 Ref.4 Ref.14

Developmental stage: Present in hair follicles at all stages of development. Ref.4

Domain: This keratin differs from all other IF proteins in lacking the C-terminal tail domain.

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.

NCBI Summary:The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
UniProt Code:P08727
NCBI GenInfo Identifier:311033484
NCBI Gene ID:3880
NCBI Accession:P08727.4
UniProt Secondary Accession:P08727,Q5XG83, Q6NW33, Q7L5M9, Q96A53, Q96FV1, Q9BYF9 Q9P1Y4, B2R874,
UniProt Related Accession:P08727
Molecular Weight:44,106 Da
NCBI Full Name:Keratin, type I cytoskeletal 19
NCBI Synonym Full Names:keratin 19
NCBI Official Symbol:KRT19  
NCBI Official Synonym Symbols:K19; CK19; K1CS  
NCBI Protein Information:keratin, type I cytoskeletal 19; CK-19; keratin-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd; 40-kDa keratin intermediate filament
UniProt Protein Name:Keratin, type I cytoskeletal 19
UniProt Synonym Protein Names:Cytokeratin-19; CK-19; Keratin-19
Protein Family:Keratin
UniProt Gene Name:KRT19  
UniProt Entry Name:K1C19_HUMAN
Anti-KRT19 Antibody (CAB0247)Western blot analysis of extracts of mouse stomach, using KRT19 antibody (CAB0247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
Anti-KRT19 Antibody (CAB0247)Immunohistochemistry of paraffin-embedded rat kidney using KRT19 antibody (CAB0247) at dilution of 1:100 (40x lens).
Anti-KRT19 Antibody (CAB0247)Immunohistochemistry of paraffin-embedded human liver cancer using KRT19 antibody (CAB0247) at dilution of 1:100 (40x lens).
Anti-KRT19 Antibody (CAB0247)Immunohistochemistry of paraffin-embedded mouse kidney using KRT19 antibody (CAB0247) at dilution of 1:100 (40x lens).
Anti-KRT19 Antibody (CAB0247)Immunofluorescence analysis of Rat rectum using KRT19 antibody (CAB0247) at dilution of 1:100. Blue: DAPI for nuclear staining.
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