Antibody Name:	Anti-KRT14 Antibody
Antibody SKU:	CAB15069
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ILLD VKTR LEQE IATY RRLL EGED AHLS SSQF SSGS QSSR DVTS SSRQ IRTK VMDV HDGK VVST HEQV LRTK N
Gene ID:	3861
Uniprot:	P02533
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	51kDa
Observed MW:	52KDa

Synonyms:

CK14, EBS3, EBS4, K14, NFJ, Cytokeratin 14, KRT14

Background:

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

UniProt Protein Function:	K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm; keratin filament; intermediate filament; cytosol; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; hair cycle; aging Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type
NCBI Summary:	This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
UniProt Code:	P02533
NCBI GenInfo Identifier:	229463044
NCBI Gene ID:	3861
NCBI Accession:	P02533.4
UniProt Secondary Accession:	P02533,P08779,
UniProt Related Accession:	P02533
Molecular Weight:	~ 52kDa
NCBI Full Name:	Keratin, type I cytoskeletal 14
NCBI Synonym Full Names:	keratin 14
NCBI Official Symbol:	KRT14
NCBI Official Synonym Symbols:	K14; NFJ; CK14; EBS3; EBS4
NCBI Protein Information:	keratin, type I cytoskeletal 14
UniProt Protein Name:	Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names:	Cytokeratin-14; CK-14; Keratin-14; K14
Protein Family:	Putative outer capsid protein
UniProt Gene Name:	KRT14
UniProt Entry Name:	K1C14_HUMAN

	Western blot analysis of extracts of various cell lines, using KRT14 Rabbit pAb (CAB15069) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Rat skin using KRT14 Rabbit pAb (CAB15069) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse skin using KRT14 Rabbit pAb (CAB15069) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Rat skin cells using KRT14 antibody (CAB15069) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Human skin cells using KRT14 antibody (CAB15069) at dilution of 1:100. Blue: DAPI for nuclear staining.