Cell Biology Antibodies 5

Anti-KRT12 Antibody (CAB14746)

(No reviews yet) Write a Review
SKU:
CAB14746
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-KRT12 Antibody
Antibody SKU:CAB14746
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human KRT12 (NP_000214.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse eye, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human KRT12 (NP_000214.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TRLL NDMR AQYE TIAE QNRK DAEA WFIE KSGE LRKE ISTN TEQL QSSK SEVT DLRR AFQN LEIE LQSQ LAMK KSLE DSLA EAEG DYCA QLSQ VQQL ISNL EAQL LQVR ADAE RQNV DHQR LLNV KARL ELEI ETYR RLLD GEAQ GDGL EESL FVTD SKSQ AQST DSSK DPTK TRKI KTVV QEMV NGEV VSSQ VQEI EELM
Gene ID:3859
Uniprot:Q99456
Cellular Location:
Calculated MW:53kDa
Observed MW:56kDa
Synonyms:KRT12, K12
Background:KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
UniProt Protein Function:K12: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD); also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Belongs to the intermediate filament family.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 17q12

Biological Process: visual perception

Disease: Corneal Dystrophy, Meesmann

NCBI Summary:KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]
UniProt Code:Q99456
NCBI GenInfo Identifier:2497269
NCBI Gene ID:3859
NCBI Accession:Q99456.1
UniProt Secondary Accession:Q99456,B2R9E0,
UniProt Related Accession:Q99456
Molecular Weight:53,511 Da
NCBI Full Name:Keratin, type I cytoskeletal 12
NCBI Synonym Full Names:keratin 12
NCBI Official Symbol:KRT12  
NCBI Official Synonym Symbols:K12  
NCBI Protein Information:keratin, type I cytoskeletal 12
UniProt Protein Name:Keratin, type I cytoskeletal 12
UniProt Synonym Protein Names:Cytokeratin-12; CK-12; Keratin-12; K12
Protein Family:Keratin
UniProt Gene Name:KRT12  
UniProt Entry Name:K1C12_HUMAN
Anti-KRT12 Antibody (CAB14746)Western blot analysis of extracts of various cell lines, using KRT12 antibody (CAB14746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
View AllClose

0 Reviews

View AllClose

Related Products