Immunology Antibodies 2

Anti-KPNA6 Antibody (CAB7363)

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SKU:
CAB7363
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-KPNA6 Antibody
Antibody SKU:CAB7363
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 277-536 of human KPNA6 (NP_036448.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BT-474, SW620, U-251MG, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 277-536 of human KPNA6 (NP_036448.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SYLS DGPN EKIQ AVID SGVC RRLV ELLM HNDY KVAS PALR AVGN IVTG DDIQ TQVI LNCS ALPC LLHL LSSP KESI RKEA CWTI SNIT AGNR AQIQ AVID ANIF PVLI EILQ KAEF RTRK EAAW AITN ATSG GTPE QIRY LVSL GCIK PLCD LLTV MDSK IVQV ALNG LENI LRLG EQEG KRSG SGVN PYCG LIEE AYGL DKIE FLQS HENQ EIYQ KAFD LIEH YFGV EDDD SSLA PQVD ETQQ QFIF QQPE APME GFQL
Gene ID:23633
Uniprot:O60684
Cellular Location:
Calculated MW:60kDa
Observed MW:60kDa
Synonyms:KPNA6, IPOA7, KPNA7
Background:Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family.
UniProt Protein Function:KPNA6: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.
UniProt Protein Details:

Protein type:Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function:protein transporter activity

Biological Process: maternal process involved in pregnancy; positive regulation of transcription from RNA polymerase II promoter; NLS-bearing substrate import into nucleus

NCBI Summary:Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq, Jul 2008]
UniProt Code:O60684
NCBI GenInfo Identifier:45476773
NCBI Gene ID:23633
NCBI Accession:O60684.1
UniProt Secondary Accession:O60684,Q5VVU3, B2RDC7, D3DPP5,
UniProt Related Accession:O60684
Molecular Weight:536
NCBI Full Name:Importin subunit alpha-7
NCBI Synonym Full Names:karyopherin alpha 6 (importin alpha 7)
NCBI Official Symbol:KPNA6  
NCBI Official Synonym Symbols:IPOA7; KPNA7  
NCBI Protein Information:importin subunit alpha-7; importin-alpha-S2; importin alpha 7 subunit; karyopherin subunit alpha-6
UniProt Protein Name:Importin subunit alpha-7
UniProt Synonym Protein Names:Karyopherin subunit alpha-6
UniProt Gene Name:KPNA6  
UniProt Entry Name:IMA7_HUMAN
Anti-KPNA6 Antibody (CAB7363)Western blot analysis of extracts of various cell lines, using KPNA6 antibody (CAB7363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-KPNA6 Antibody (CAB7363)Immunohistochemistry of paraffin-embedded human kidney using KPNA6 antibody (CAB7363) at dilution of 1:100 (40x lens).
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