Immunology Antibodies 2

Anti-KPNA5 Antibody (CAB7331)

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SKU:
CAB7331
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-KPNA5 Antibody
Antibody SKU:CAB7331
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA5 (NP_002260.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, SKOV3, HepG2, NCI-H460, Mouse testis, Mouse liver, Mouse heart, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA5 (NP_002260.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDAM ASPG KDNY RMKS YKNK ALNP QEMR RRRE EEGI QLRK QKRE EQLF KRRN VYLP RNDE SMLE SPIQ DPDI SSTV PIPE EEVV TTDM VQMI FSNN ADQQ LTAT QKFR KLLS KEPN PPID QVIQ KPGV VQRF VKFL ERNE NCTL QFEA AWAL TNIA SGTF LHTK VVIE TGAV PIFI KLLN SEHE DVQE QAVW ALGN IAGD NAEC RDFV LNCE ILPP LLEL LTNS NRLT TTRN AVWA LSNL
Gene ID:3841
Uniprot:O15131
Cellular Location:Cytoplasm
Calculated MW:60kDa
Observed MW:60kDa
Synonyms:KPNA5, IPOA6, SRP6
Background:The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus.
UniProt Protein Function:KPNA5: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non- classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Belongs to the importin alpha family.
UniProt Protein Details:

Protein type:Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function:protein binding; protein transporter activity

Biological Process: cytokine and chemokine mediated signaling pathway; NLS-bearing substrate import into nucleus

NCBI Summary:The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]
UniProt Code:O15131
NCBI GenInfo Identifier:239938645
NCBI Gene ID:3841
NCBI Accession:O15131.2
UniProt Related Accession:O15131
Molecular Weight:Predicted Molecular Mass: 43.2kDaAccurate Molecular Mass: 43kDa
NCBI Full Name:Importin subunit alpha-6
NCBI Synonym Full Names:karyopherin subunit alpha 5
NCBI Official Symbol:KPNA5  
NCBI Official Synonym Symbols:SRP6; IPOA6  
NCBI Protein Information:importin subunit alpha-6
UniProt Protein Name:Importin subunit alpha-6
UniProt Synonym Protein Names:Karyopherin subunit alpha-5
UniProt Gene Name:KPNA5  
UniProt Entry Name:IMA6_HUMAN
Anti-KPNA5 Antibody (CAB7331)Western blot analysis of extracts of various cell lines, using KPNA5 antibody (CAB7331) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.
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