Antibody Name:	Anti-KPNA3 Antibody
Antibody SKU:	CAB8347
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human KPNA3 (NP_002258.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Jurkat, A-549, U-251MG, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human KPNA3 (NP_002258.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEN PSLE NHRI KSFK NKGR DVET MRRH RNEV TVEL RKNK RDEH LLKK RNVP QEES LEDS DVDA DFKA QNVT LEAI LQNA TSDN PVVQ LSAV QAAR KLLS SDRN PPID DLIK SGIL PILV KCLE RDDN PSLQ FEAA WALT NIAS GTSA QTQA VVQS NAVP LFLR LLRS PHQN VCEQ AVWA LGNI IGDG PQCR DYVI SLGV VKPL LSFI SP
Gene ID:	3839
Uniprot:	O00505
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	57kDa
Observed MW:	55kDa

Synonyms:

KPNA3, IPOA4, SRP1, SRP1gamma, SRP4, hSRP1

Background:

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import.

UniProt Protein Function:	KPNA3: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. Belongs to the importin alpha family.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Chaperone; Karyopherin; Nuclear import Chromosomal Location of Human Ortholog: 13q14.3 Cellular Component: nucleoplasm; nuclear pore; cytosol Molecular Function:protein C-terminus binding; protein binding; nuclear localization sequence binding; protein transporter activity Biological Process: entry of virus into host cell; cytokine and chemokine mediated signaling pathway; protein complex assembly; NLS-bearing substrate import into nucleus
NCBI Summary:	The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq, Jan 2009]
UniProt Code:	O00505
NCBI GenInfo Identifier:	34485722
NCBI Gene ID:	3839
NCBI Accession:	NP_002258
UniProt Related Accession:	O00505
Molecular Weight:	58kDa
NCBI Full Name:	importin subunit alpha-4
NCBI Synonym Full Names:	karyopherin subunit alpha 3
NCBI Official Symbol:	KPNA3
NCBI Official Synonym Symbols:	SRP1; SRP4; IPOA4; hSRP1; SRP1gamma
NCBI Protein Information:	importin subunit alpha-4
UniProt Protein Name:	Importin subunit alpha-4
UniProt Synonym Protein Names:	Importin alpha Q2; Qip2; Karyopherin subunit alpha-3; SRP1-gamma
UniProt Gene Name:	KPNA3
UniProt Entry Name:	IMA4_HUMAN

	Western blot analysis of extracts of various cell lines, using KPNA3 antibody (CAB8347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat kidney using KPNA3 Rabbit pAb (CAB8347) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA3 Rabbit pAb (CAB8347) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using KPNA3 Rabbit pAb (CAB8347) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat stomach using KPNA3 Rabbit pAb (CAB8347) at dilution of 1:100 (40x lens).