Antibody Name:	Anti-KPNA1 Antibody
Antibody SKU:	CAB1742
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KPNA1 (NP_002255.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, K-562, HepG2, HeLa, A-549, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KPNA1 (NP_002255.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTP GKEN FRLK SYKN KSLN PDEM RRRR EEEG LQLR KQKR EEQL FKRR NVAT AEEE TEEE VMSD GGFH EAQI SNME MAPG GVIT SDMI EMIF SKSP EQQL SATQ KFRK LLSK EPNP PIDE VIST PGVV ARFV EFLK RKEN CTLQ FESA WVLT NIAS GNSL QTRI VIQA GAVP IFIE LLSS EFED VQEQ AVWA LGNI AGDS TMCR DYVL DCNI LPPL LQLF SKQN RLTM TRNA VWAL SNLC RGKS PPPE FAKV SPCL NVLS WLLF VSDT DVLA DACW ALSY LSDG PNDK IQAV IDAG VCRR
Gene ID:	3836
Uniprot:	P52294
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	60kDa
Observed MW:	60kDa/70kDa

Synonyms:

KPNA1, IPOA5, NPI-1, RCH2, SRP1

Background:

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	KPNA1: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Belongs to the importin alpha family.
UniProt Protein Details:	Protein type:Nuclear import; Karyopherin; Adaptor/scaffold Chromosomal Location of Human Ortholog: 3q21 Cellular Component: nucleoplasm; dendrite; cytoplasm; nuclear pore; cytosol; nucleus Molecular Function:protein binding; nuclear localization sequence binding; protein transporter activity Biological Process: viral reproduction; positive regulation of protein import into nucleus; apoptosis; regulation of DNA recombination; cytokine and chemokine mediated signaling pathway; DNA fragmentation during apoptosis; NLS-bearing substrate import into nucleus; viral infectious cycle; cell structure disassembly during apoptosis
NCBI Summary:	The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:	P52294
NCBI GenInfo Identifier:	296439328
NCBI Gene ID:	3836
NCBI Accession:	P52294.3
UniProt Related Accession:	P52294
Molecular Weight:
NCBI Full Name:	Importin subunit alpha-5
NCBI Synonym Full Names:	karyopherin subunit alpha 1
NCBI Official Symbol:	KPNA1
NCBI Official Synonym Symbols:	RCH2; SRP1; IPOA5; NPI-1
NCBI Protein Information:	importin subunit alpha-5
UniProt Protein Name:	Importin subunit alpha-5
UniProt Synonym Protein Names:	Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-betaImportin subunit alpha-5, N-terminally processed
Protein Family:	Integral inner nuclear membrane protein
UniProt Gene Name:	KPNA1
UniProt Entry Name:	IMA5_HUMAN

	Western blot analysis of extracts of various cell lines, using KPNA1 antibody (CAB1742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human lung cancer using KPNA1 antibody (CAB1742) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using KPNA1 antibody (CAB1742) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using KPNA1 antibody (CAB1742).