Cell Cycle Antibodies 1

Anti-KNTC1 Antibody (CAB13064)

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SKU:
CAB13064
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-KNTC1 Antibody
Antibody SKU:CAB13064
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1100 of human KNTC1 (NP_055523.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1100 of human KNTC1 (NP_055523.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IDLI DREQ GEDC LLLL KSLP PAEA EKTA ERVI IWAR LALQ EEPD HSKE GKAW RMSV AKTS VDIL KILC DIQK DNLQ KKDE CEEM LKLF KEVA SLQE NFEV FLSF EDYS NSSL VADL REQH IKAH EVAQ AKHK PGST PEPI AAEV RSPS MESK LHRQ ALAL QMSK QELE AELT LRAL KDGN IKTA LKKC SDLF KYHC NADT G
Gene ID:9735
Uniprot:P50748
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle
Calculated MW:51kDa/250kDa
Observed MW:250kDa
Synonyms:KNTC1, ROD
Background:This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein.
UniProt Protein Function:KNTC1: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: actin cytoskeleton; cytosol; kinetochore microtubule; nucleus; plasma membrane; spindle pole

Molecular Function:protein binding

Biological Process: cell division; mitotic cell cycle checkpoint; protein complex assembly; regulation of exit from mitosis; sister chromatid cohesion

NCBI Summary:This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008]
UniProt Code:P50748
NCBI GenInfo Identifier:1519244775
NCBI Gene ID:9735
NCBI Accession:NM_014708.6
UniProt Related Accession:P50748
Molecular Weight:
NCBI Full Name:Homo sapiens kinetochore associated 1 (KNTC1), mRNA
NCBI Synonym Full Names:kinetochore associated 1
NCBI Official Symbol:KNTC1  
NCBI Official Synonym Symbols:ROD  
NCBI Protein Information:kinetochore-associated protein 1
UniProt Protein Name:Kinetochore-associated protein 1
UniProt Synonym Protein Names:Rough deal homolog; HsROD; Rod; hRod
Protein Family:Kinetochore-associated protein
UniProt Gene Name:KNTC1  
Anti-KNTC1 Antibody (CAB13064)Western blot analysis of extracts of 293T cells, using KNTC1 antibody (CAB13064) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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