Cardiovascular Antibodies
Anti-KNG1 Antibody (CAB1670)
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- SKU:
- CAB1670
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-KNG1 Antibody |
| Antibody SKU: | CAB1670 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human KNG1 (NP_000884.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human KNG1 (NP_000884.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PIST QSPD LEPI LRHG IQYF NNNT QHSS LFML NEVK RAQR QVVA GLNF RITY SIVQ TNCS KENF LFLT PDCK SLWN GDTG ECTD NAYI DIQL RIAS FSQN CDIY PGKD FVQP PTKI CVGC PRDI PTNS PELE ETLT HTIT KLNA ENNA TFYF KIDN VKKA RVQV VAGK KYFI DFVA RETT CSKE SNEE LTES CETK KLGQ SLDC NAEV YVVP WEKK IYPT VNCQ PLGM ISLM KRPP GFSP FRSS RIGE IKEE TTSH LRSC EYKG RPPK AGAE PASE REVS |
| Gene ID: | 3827 |
| Uniprot: | P01042 |
| Cellular Location: | Secreted, extracellular space |
| Calculated MW: | 43kDa/47kDa/71kDa |
| Observed MW: | 68kDa |
| Synonyms: | KNG1, BDK, BK, KNG |
| Background: | This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Contractile; Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis; Inhibitor Chromosomal Location of Human Ortholog: 3q27 Cellular Component: extracellular space; extracellular region; plasma membrane Molecular Function:heparin binding; protein binding; zinc ion binding; cysteine protease inhibitor activity; receptor binding Biological Process: negative regulation of proteolysis; platelet activation; elevation of cytosolic calcium ion concentration; smooth muscle contraction; platelet degranulation; positive regulation of apoptosis; negative regulation of blood coagulation; negative regulation of cell adhesion; blood coagulation; inflammatory response; vasodilation; blood coagulation, intrinsic pathway Disease: High Molecular Weight Kininogen Deficiency |
| NCBI Summary: | This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014] |
| UniProt Code: | P01042 |
| NCBI GenInfo Identifier: | 124056474 |
| NCBI Gene ID: | 3827 |
| NCBI Accession: | P01042.2 |
| UniProt Secondary Accession: | P01042,P01043, Q53EQ0, Q6PAU9, Q7M4P1, A8K474, B2RCR2 C9JEX1, |
| UniProt Related Accession: | P01042 |
| Molecular Weight: | 644 |
| NCBI Full Name: | Kininogen-1 |
| NCBI Synonym Full Names: | kininogen 1 |
| NCBI Official Symbol: | KNG1 |
| NCBI Official Synonym Symbols: | BK; BDK; KNG |
| NCBI Protein Information: | kininogen-1; HMWK; bradykinin; fitzgerald factor; high molecular weight kininogen; alpha-2-thiol proteinase inhibitor; williams-Fitzgerald-Flaujeac factor |
| UniProt Protein Name: | Kininogen-1 |
| UniProt Synonym Protein Names: | Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; HMWK; Williams-Fitzgerald-Flaujeac factorCleaved into the following 6 chains:Kininogen-1 heavy chain; T-kininAlternative name(s):Ile-Ser-Bradykinin |
| Protein Family: | Kininogen |
| UniProt Gene Name: | KNG1 |
| UniProt Entry Name: | KNG1_HUMAN |
 | Western blot analysis of extracts of HT-29 cells, using KNG1 antibody (CAB1670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using KNG1 Rabbit pAb (CAB1670) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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