KO Validated Antibodies 1

Anti-KMT5C Antibody (CAB16235)[KO Validated]

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SKU:
CAB16235
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-KMT5C Antibody [KO Validated]
Antibody SKU:CAB16235
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human KMT5C (NP_116090.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, HeLa, K-562, SH-SY5Y, Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human KMT5C (NP_116090.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FLPE SGFT ILPC TRYS METN GAKI VSTR AWKK NEKL ELLV GCIA ELRE ADEG LLRA GEND FSIM YSTR KRSA QLWL GPAA FINH DCKP NCKF VPAD GNAA C
Gene ID:84787
Uniprot:Q86Y97
Cellular Location:Chromosome, Nucleus
Calculated MW:10kDa/52kDa
Observed MW:52kDa
Synonyms:KMT5C, SUV420H2, Suv4-20h2
Background:SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20).
UniProt Protein Function:SUV420H2: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: centric heterochromatin; nuclear heterochromatin; nucleoplasm

Molecular Function:protein binding

NCBI Summary:SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).[supplied by OMIM, Dec 2009]
UniProt Code:Q86Y97
NCBI GenInfo Identifier:74727906
NCBI Gene ID:84787
NCBI Accession:Q86Y97.1
UniProt Secondary Accession:Q86Y97,Q8WZ10, Q9BRZ6,
UniProt Related Accession:Q86Y97
Molecular Weight:10,821 Da
NCBI Full Name:Histone-lysine N-methyltransferase KMT5C
NCBI Synonym Full Names:lysine methyltransferase 5C
NCBI Official Symbol:KMT5C  
NCBI Official Synonym Symbols:SUV420H2  
NCBI Protein Information:histone-lysine N-methyltransferase KMT5C
UniProt Protein Name:Histone-lysine N-methyltransferase KMT5C
UniProt Synonym Protein Names:Lysine N-methyltransferase 5C; Lysine-specific methyltransferase 5C
UniProt Gene Name:KMT5C  
UniProt Entry Name:KMT5C_HUMAN
Anti-KMT5C Antibody [KO Validated] (CAB16235)Western blot analysis of extracts of various cell lines, using KMT5C antibody (CAB16235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-KMT5C Antibody [KO Validated] (CAB16235)Western blot analysis of extracts from normal (control) and KMT5C Rabbit pAb knockout (KO) 293T cells, using KMT5C Rabbit pAb antibody (CAB16235) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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