Cell Cycle Antibodies 2
Anti-KMT5A / SETD8 Antibody (CAB4136)
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- SKU:
- CAB4136
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-KMT5A / SETD8 Antibody |
| Antibody SKU: | CAB4136 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human KMT5A / SETD8 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, U-87MG, Mouse brain, Mouse heart |
| Immunogen: | A synthesized peptide derived from human KMT5A / SETD8 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 387893 |
| Uniprot: | Q9NQR1 |
| Cellular Location: | |
| Calculated MW: | 50kDa |
| Observed MW: | 49KDa |
| Synonyms: | PR-Set7, SET07, SET8, SETD8 |
| Background: | The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation. [provided by RefSeq, May 2016] |
| UniProt Protein Function: | SETD8: a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Methylates K20 of histone H4 (H4K20me1), a specific tag for epigenetic transcriptional repression. SETD8 protein and H4K20me1 levels are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. Interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Nucleosomes are preferred as substrate compared to free histones. Methylates p53K382, leading to repression of the pro-apoptotic and checkpoint activation functions of p53. SET8 expression levels decrease in response to DNA damage, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4K20 and p53K382 appear to be important for the functions of SET8 in S phase. Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Two isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleoplasm; nucleolus; chromosome; nucleus Molecular Function:protein binding; p53 binding; protein-lysine N-methyltransferase activity; transcription corepressor activity; histone-lysine N-methyltransferase activity Biological Process: mitosis; transcription, DNA-dependent; cell division; peptidyl-lysine mono-methylation; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of DNA damage response, signal transduction by p53 class mediator |
| UniProt Code: | Q9NQR1 |
| NCBI GenInfo Identifier: | 25091219 |
| NCBI Gene ID: | 387893 |
| NCBI Accession: | Q9NQR1.3 |
| UniProt Secondary Accession: | Q9NQR1,Q86W83, Q8TD09, A8K9D0, |
| UniProt Related Accession: | Q9NQR1 |
| Molecular Weight: | 393 |
| NCBI Full Name: | N-lysine methyltransferase SETD8 |
| NCBI Synonym Full Names: | SET domain containing (lysine methyltransferase) 8 |
| NCBI Official Symbol: | SETD8 |
| NCBI Official Synonym Symbols: | SET8; KMT5A; SET07; PR-Set7 |
| NCBI Protein Information: | N-lysine methyltransferase SETD8; PR/SET07; H4-K20-HMTase SETD8; lysine N-methyltransferase 5A; SET domain-containing protein 8; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; histone-lysine N-methyltransferase SETD8; H4-K20-spec |
| UniProt Protein Name: | N-lysine methyltransferase SETD8 |
| UniProt Synonym Protein Names: | H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8 (EC:2.1.1.43); Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8 |
| Protein Family: | N-lysine methyltransferase |
| UniProt Gene Name: | SETD8 |
| UniProt Entry Name: | SETD8_HUMAN |
 | Western blot - KMT5A / SETD8 Rabbit mAb (CAB4136) |
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