Name: Anti-KMT2E Antibody (CAB6142)
Brand: Cell Cycle Antibodies 1
SKU: CAB6142
Availability: OutOfStock

Antibody Name:	Anti-KMT2E Antibody
Antibody SKU:	CAB6142
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human KMT2E (NP_061152.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, 293T, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human KMT2E (NP_061152.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VELY TAFQ HTPT SITL TASR VSKV NDKR RKKS GEKE QHIS KCKK AFRE GSRK SSRV KGSA PEID PSSD GSNF GWET KIKA WMDR YEEA NNNQ YSEG VQRE AQRI ALRL GNGN DKKE MNKS DLNT NNLL FKPP VESH IQKN KKIL KSAK DLPP DALI IEYR GKFM LREQ FEAN GYFF KRPY PFVL FYSK FHGL EMCV DART FGNE ARFI RRSC TPNA EVRH EIQD GTIH LYIY SIHS IPKG TEIT IAFD FDYG NCKY KVDC ACLK ENPE CPVL KRSS ESME NINS GYET RRKK GKKD KDIS K
Gene ID:	55904
Uniprot:	Q8IZD2
Cellular Location:	Cell membrane, Cytoplasm, Nucleus speckle, Peripheral membrane protein
Calculated MW:	68kDa/98kDa/131kDa/180-204kDa
Observed MW:	95-140kDa

Synonyms:	KMT2E, HDCMC04P, MLL5, NKp44L
Background:	This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized.

UniProt Protein Function:	MLL5: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.43; Transcription, coactivator/corepressor; Methyltransferase, protein lysine; Cell cycle regulation; Methyltransferase Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: nucleoplasm; intercellular bridge; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nuclear speck Molecular Function:protein binding; enzyme binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity Biological Process: retinoic acid receptor signaling pathway; establishment and/or maintenance of chromatin architecture; neutrophil activation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; histone H3-K4 methylation; DNA methylation; neutrophil mediated immunity; erythrocyte differentiation; cell cycle arrest
NCBI Summary:	This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IZD2
NCBI GenInfo Identifier:	74723669
NCBI Gene ID:	55904
NCBI Accession:	Q8IZD2.1
UniProt Secondary Accession:	Q8IZD2,Q6P5Y2, Q6PKG4, Q6T316, Q86TI3, Q86W12, Q86WG0 Q86WL2, Q8IV78, B6ZDE4, B6ZDM3, M4K8J3,
UniProt Related Accession:	Q8IZD2
Molecular Weight:	1858
NCBI Full Name:	Histone-lysine N-methyltransferase 2E
NCBI Synonym Full Names:	lysine (K)-specific methyltransferase 2E
NCBI Official Symbol:	KMT2E
NCBI Official Synonym Symbols:	MLL5; NKp44L; HDCMC04P
NCBI Protein Information:	histone-lysine N-methyltransferase 2E; histone-lysine N-methyltransferase MLL5; myeloid/lymphoid or mixed-lineage leukemia protein 5; myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
UniProt Protein Name:	Histone-lysine N-methyltransferase 2E
UniProt Synonym Protein Names:	Myeloid/lymphoid or mixed-lineage leukemia protein 5
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	KMT2E
UniProt Entry Name:	KMT2E_HUMAN

	Western blot analysis of extracts of various cell lines, using KMT2E Antibody (CAB6142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat ovary using KMT2E antibody (CAB6142) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using KMT2E antibody (CAB6142) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using KMT2E antibody (CAB6142) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of mouse brain using KMT2E antibody (CAB6142) at dilution of 1:100. Blue: DAPI for nuclear staining.