Antibody Name:	Anti-KLRC4 Antibody
Antibody SKU:	CAB14807
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-55 of human KLRC4 (NP_038459.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, NCI-H460, 293T, Mouse lung, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-55 of human KLRC4 (NP_038459.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNKQ RGTY SEVS LAQD PKRQ QRKL KGNK ISIS GTKQ EIFQ VELN LQNA SSDH QGN
Gene ID:	8302
Uniprot:	O43908
Cellular Location:	Membrane, Single-pass type II membrane protein
Calculated MW:	18kDa
Observed MW:	14kDa

Synonyms:

KLRC4, NKG2-F, NKG2F

Background:

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene is a member of the NKG2 group of genes that are expressed primarily in natural killer (NK) cells. These family members encode transmembrane proteins that are characterized by a type II membrane orientation (have an extracellular C-terminus) and the presence of a C-type lectin domain. This family member is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. Read-through transcription exists between this gene and the downstream KLRK1 (killer cell lectin-like receptor subfamily K, member 1) family member.

UniProt Protein Function:	KLRC4: May play a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 12p13.2-p12.3 Cellular Component: integral to membrane Biological Process: cellular defense response
NCBI Summary:	Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene is a member of the NKG2 group of genes that are expressed primarily in natural killer (NK) cells. These family members encode transmembrane proteins that are characterized by a type II membrane orientation (have an extracellular C-terminus) and the presence of a C-type lectin domain. This family member is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. Read-through transcription exists between this gene and the downstream KLRK1 (killer cell lectin-like receptor subfamily K, member 1) family member. [provided by RefSeq, Dec 2010]
UniProt Code:	O43908
NCBI GenInfo Identifier:	7710124
NCBI Gene ID:	8302
NCBI Accession:	NP_038459.1
UniProt Secondary Accession:	O43908,O60851,
UniProt Related Accession:	O43908
Molecular Weight:	18,234 Da
NCBI Full Name:	NKG2-F type II integral membrane protein
NCBI Synonym Full Names:	killer cell lectin like receptor C4
NCBI Official Symbol:	KLRC4
NCBI Official Synonym Symbols:	NKG2F; NKG2-F
NCBI Protein Information:	NKG2-F type II integral membrane protein
UniProt Protein Name:	NKG2-F type II integral membrane protein
UniProt Synonym Protein Names:	NK cell receptor F; NKG2-F-activating NK receptor
Protein Family:	NKG2-F type II integral membrane protein
UniProt Gene Name:	KLRC4
UniProt Entry Name:	NKG2F_HUMAN

	Western blot analysis of extracts of various cell lines, using KLRC4 antibody (CAB14807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of Jurkat cells using KLRC4 Polyclonal Antibody (CAB14807) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse spleen using KLRC4 Polyclonal Antibody (CAB14807) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.