Cardiovascular Antibodies

Anti-KLKB1 Antibody (CAB13322)

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SKU:
CAB13322
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-KLKB1 Antibody
Antibody SKU:CAB13322
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human KLKB1 (NP_000883.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, BxPC-3, Mouse liver, Mouse pancreas , Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human KLKB1 (NP_000883.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GCLT QLYE NAFF RGGD VASM YTPN AQYC QMRC TFHP RCLL FSFL PASS INDM EKRF GCFL KDSV TGTL PKVH RTGA VSGH SLKQ CGHQ ISAC HRDI YKGV DMRG VNFN VSKV SSVE ECQK RCTS NIRC QFFS YATQ TFHK AEYR NNCL LKYS PGGT PTAI KVLS NVES GFSL KPCA LSEI GCHM NIFQ HLAF SDVD VARV LTPD AFVC RTIC TYHP NCLF FTFY TNVW KIES QRNV CLLK TSES GTPS SSTP QENT ISGY SLLT CKRT LPEP CHSK IYPG V
Gene ID:3818
Uniprot:P03952
Cellular Location:Secreted
Calculated MW:71kDa
Observed MW:71kDa
Synonyms:KLKB1, KLK3, PKK, PKKD, PPK
Background:This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
UniProt Protein Details:

Protein type:Secreted; EC 3.4.21.34; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function:protein binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; fibrinolysis; positive regulation of fibrinolysis; zymogen activation; proteolysis; blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation; plasminogen activation

Disease: Prekallikrein Deficiency

NCBI Summary:Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]
UniProt Code:P03952
NCBI GenInfo Identifier:125184
NCBI Gene ID:3818
NCBI Accession:P03952.1
UniProt Secondary Accession:P03952,Q17RE8, Q17RE9, Q4W5C3, A6NH96, B2R8H9,
UniProt Related Accession:P03952
Molecular Weight:71,370 Da
NCBI Full Name:Plasma kallikrein
NCBI Synonym Full Names:kallikrein B, plasma (Fletcher factor) 1
NCBI Official Symbol:KLKB1  
NCBI Official Synonym Symbols:PPK; KLK3  
NCBI Protein Information:plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain
UniProt Protein Name:Plasma kallikrein
UniProt Synonym Protein Names:Fletcher factor; Kininogenin; Plasma prekallikrein
Protein Family:Protein kinase
UniProt Gene Name:KLKB1  
UniProt Entry Name:KLKB1_HUMAN
Anti-KLKB1 Antibody (CAB13322)Western blot analysis of extracts of various cell lines, using KLKB1 Rabbit pAb (CAB13322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-KLKB1 Antibody (CAB13322)Immunohistochemistry of paraffin-embedded mouse testis using KLKB1 antibody (CAB13322) at dilution of 1:200 (40x lens).
Anti-KLKB1 Antibody (CAB13322)Immunohistochemistry of paraffin-embedded mouse lung using KLKB1 antibody (CAB13322) at dilution of 1:200 (40x lens).
Anti-KLKB1 Antibody (CAB13322)Immunofluorescence analysis of HeLa cells using KLKB1 antibody (CAB13322). Blue: DAPI for nuclear staining.
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