Antibody Name:	Anti-KLK4 Antibody
Antibody SKU:	CAB6642
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-254 of human KLK4 (NP_004908.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-254 of human KLK4 (NP_004908.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QIIN GEDC SPHS QPWQ AALV MENE LFCS GVLV HPQW VLSA AHCF QNSY TIGL GLHS LEAD QEPG SQMV EASL SVRH PEYN RPLL ANDL MLIK LDES VSES DTIR SISI ASQC PTAG NSCL VSGW GLLA NGRM PTVL QCVN VSVV SEEV CSKL YDPL YHPS MFCA GGGQ DQKD SCNG DSGG PLIC NGYL QGLV SFGK APCG QVGV PGVY TNLC KFTE WIEK TVQA S
Gene ID:	9622
Uniprot:	Q9Y5K2
Cellular Location:	Secreted
Calculated MW:	11kDa/27kDa
Observed MW:	27kDa

Synonyms:

KLK4, AI2A1, ARM1, EMSP, EMSP1, KLK-L1, PRSS17, PSTS, kallikrein

Background:

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In some tissues its expression is hormonally regulated. The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation of enamel proteins. Several transcript variants encoding different proteins have been found for this gene.

UniProt Protein Function:	KLK4: Involved in enamel formation. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1). AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase S1 family. Kallikrein subfamily.
UniProt Protein Details:	Protein type:Secreted, signal peptide; EC 3.4.21.-; Secreted; Protease Chromosomal Location of Human Ortholog: 19q13.41 Cellular Component: extracellular region Molecular Function:serine-type peptidase activity; serine-type endopeptidase activity; metal ion binding Biological Process: extracellular matrix disassembly; protein catabolic process; proteolysis Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia1
NCBI Summary:	Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In some tissues its expression is hormonally regulated. The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation of enamel proteins. Several transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:	Q9Y5K2
NCBI GenInfo Identifier:	317373372
NCBI Gene ID:	9622
NCBI Accession:	Q9Y5K2.2
UniProt Secondary Accession:	Q9Y5K2,Q4VB16, Q96RU5, Q9GZL6, Q9UBJ6,
UniProt Related Accession:	Q9Y5K2
Molecular Weight:	254
NCBI Full Name:	Kallikrein-4
NCBI Synonym Full Names:	kallikrein-related peptidase 4
NCBI Official Symbol:	KLK4
NCBI Official Synonym Symbols:	ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
NCBI Protein Information:	kallikrein-4; prostase; serine protease 17; kallikrein-like protein 1; androgen-regulated message 1; enamel matrix serine protease 1; enamel matrix serine proteinase 1
UniProt Protein Name:	Kallikrein-4
UniProt Synonym Protein Names:	Enamel matrix serine proteinase 1; Kallikrein-like protein 1; KLK-L1; Prostase; Serine protease 17
Protein Family:	Kallikrein
UniProt Gene Name:	KLK4
UniProt Entry Name:	KLK4_HUMAN

	Western blot analysis of extracts of various cell lines, using KLK4 Antibody (CAB6642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of MCF7 cells using KLK4 antibody (CAB6642). Blue: DAPI for nuclear staining.