Cell Biology Antibodies 8

Anti-KLK3 Antibody (CAB2052)

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SKU:
CAB2052
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KLK3 Antibody
Antibody SKU:CAB2052
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLK3 (NP_001639.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human
Positive Samples:LNCaP
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLK3 (NP_001639.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWVP VVFL TLSV TWIG AAPL ILSR IVGG WECE KHSQ PWQV LVAS RGRA VCGG VLVH PQWV LTAA HCIR NKSV ILLG RHSL FHPE DTGQ VFQV SHSF PHPL
Gene ID:354
Uniprot:P07288
Cellular Location:Secreted
Calculated MW:7kDa/23kDa/25kDa/26kDa/28kDa
Observed MW:34KDa
Synonyms:KLK3, APS, KLK2A1, PSA, hK3
Background:Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
UniProt Protein Function:PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.
UniProt Protein Details:

Protein type:Protease; Secreted, signal peptide; EC 3.4.21.77; Secreted

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function:protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis

NCBI Summary:Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:P07288
NCBI GenInfo Identifier:130989
NCBI Gene ID:354
NCBI Accession:P07288.2
UniProt Secondary Accession:P07288,Q15096, Q16272, Q86TG8, Q8IXI4, C9JXH3, G3V0H4 G3XAE3,
UniProt Related Accession:P07288
Molecular Weight:28,741 Da
NCBI Full Name:Prostate-specific antigen
NCBI Synonym Full Names:kallikrein-related peptidase 3
NCBI Official Symbol:KLK3  
NCBI Official Synonym Symbols:APS; PSA; hK3; KLK2A1  
NCBI Protein Information:prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
UniProt Protein Name:Prostate-specific antigen
UniProt Synonym Protein Names:Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
Protein Family:Prostate-specific antigen
UniProt Gene Name:KLK3  
UniProt Entry Name:KLK3_HUMAN
Anti-KLK3 Antibody (CAB2052)Western blot analysis of extracts of LNCaP cells, using KLK3 antibody (CAB2052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-KLK3 Antibody (CAB2052)Immunohistochemistry of paraffin-embedded human prostate cancer using KLK3 Rabbit pAb (CAB2052) at dilution of 1:100 (40x lens).
Anti-KLK3 Antibody (CAB2052)Immunofluorescence analysis of LnCap cells using KLK3 Polyclonal Antibody (CAB2052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-KLK3 Antibody (CAB2052)Immunoprecipitation analysis of 300ug extracts of LNCaP cells using 3ug KLK3 antibody (CAB2052). Western blot was performed from the immunoprecipitate using KLK3 antibody (CAB2052) at a dilition of 1:2000.
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