Antibody Name:	Anti-KLK10 Antibody
Antibody SKU:	CAB6398
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-276 of human KLK10 (NP_001070968.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse brain, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-276 of human KLK10 (NP_001070968.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLPQ NDTR LDPE AYGS PCAR GSQP WQVS LFNG LSFH CAGV LVDQ SWVL TAAH CGNK PLWA RVGD DHLL LLQG EQLR RTTR SVVH PKYH QGSG PILP RRTD EHDL MLLK LARP VVLG PRVR ALQL PYRC AQPG DQCQ VAGW GTTA ARRV KYNK GLTC SSIT ILSP KECE VFYP GVVT NNMI CAGL DRGQ DPCQ SDSG GPLV CDET LQGI LSWG VYPC GSAQ HPAV YTQI CKYM SWIN KVIR SN
Gene ID:	5655
Uniprot:	O43240
Cellular Location:	Secreted
Calculated MW:	30kDa
Observed MW:	30kDa

Synonyms:

KLK10, NES1, PRSSL1

Background:

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein.

UniProt Protein Function:	KLK10: Has a tumor-suppressor role for NES1 in breast and prostate cancer. Belongs to the peptidase S1 family. Kallikrein subfamily.
UniProt Protein Details:	Protein type:Secreted; Tumor suppressor; Protease; Secreted, signal peptide; EC 3.4.21.- Chromosomal Location of Human Ortholog: 19q13 Molecular Function:serine-type peptidase activity
NCBI Summary:	Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:	O43240
NCBI GenInfo Identifier:	317373432
NCBI Gene ID:	5655
NCBI Accession:	O43240.3
UniProt Secondary Accession:	O43240,Q53YL3, Q99920, Q9GZW9, A6NC12,
UniProt Related Accession:	O43240
Molecular Weight:	30,170 Da
NCBI Full Name:	Kallikrein-10
NCBI Synonym Full Names:	kallikrein related peptidase 10
NCBI Official Symbol:	KLK10
NCBI Official Synonym Symbols:	NES1; PRSSL1
NCBI Protein Information:	kallikrein-10
UniProt Protein Name:	Kallikrein-10
UniProt Synonym Protein Names:	Normal epithelial cell-specific 1; Protease serine-like 1
Protein Family:	Kallikrein
UniProt Gene Name:	KLK10
UniProt Entry Name:	KLK10_HUMAN

	Western blot analysis of extracts of various cell lines, using KLK10 antibody (CAB6398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast using KLK10 Antibody (CAB6398) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using KLK10 antibody (CAB6398). Blue: DAPI for nuclear staining.