Cell Cycle Antibodies 1
Anti-KLHL9 Antibody (CAB10149)
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- SKU:
- CAB10149
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-KLHL9 Antibody |
| Antibody SKU: | CAB10149 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KLHL9 (NP_061335.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, A-549, HT-1080, MCF-7, 22Rv1, Mouse kidney, Mouse lung, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KLHL9 (NP_061335.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKVS LGNG EMGV SAHL QPCK AGTT RFFT SNTH SSVV LQGF DQLR IEGL LCDV TLVP GDGD EIFP VHRA MMAS ASDY FKAM FTGG MKEQ DLMC IKLH GVNK |
| Gene ID: | 55958 |
| Uniprot: | Q9P2J3 |
| Cellular Location: | |
| Calculated MW: | 69kDa |
| Observed MW: | 75kDa |
| Synonyms: | KLHL9 |
| Background: |
| UniProt Protein Function: | KLHL9: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 9p22 Cellular Component: midbody Molecular Function:ubiquitin-protein ligase activity Biological Process: mitosis; cytokinesis; protein ubiquitination |
| UniProt Code: | Q9P2J3 |
| NCBI GenInfo Identifier: | 51338828 |
| NCBI Gene ID: | 55958 |
| NCBI Accession: | Q9P2J3.2 |
| UniProt Secondary Accession: | Q9P2J3,Q8TCQ2, |
| UniProt Related Accession: | Q9P2J3 |
| Molecular Weight: | 69,429 Da |
| NCBI Full Name: | Kelch-like protein 9 |
| NCBI Synonym Full Names: | kelch-like family member 9 |
| NCBI Official Symbol: | KLHL9 |
| NCBI Protein Information: | kelch-like protein 9; kelch-like 9 |
| UniProt Protein Name: | Kelch-like protein 9 |
| Protein Family: | Kelch-like protein |
| UniProt Gene Name: | KLHL9 |
| UniProt Entry Name: | KLHL9_HUMAN |
 | Western blot analysis of extracts of 293T cells, using KLHL9 antibody (CAB10149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens). |
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