Cell Cycle Antibodies 1

Anti-KLHL9 Antibody (CAB10149)

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SKU:
CAB10149
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-KLHL9 Antibody
Antibody SKU:CAB10149
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KLHL9 (NP_061335.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, A-549, HT-1080, MCF-7, 22Rv1, Mouse kidney, Mouse lung, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KLHL9 (NP_061335.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKVS LGNG EMGV SAHL QPCK AGTT RFFT SNTH SSVV LQGF DQLR IEGL LCDV TLVP GDGD EIFP VHRA MMAS ASDY FKAM FTGG MKEQ DLMC IKLH GVNK
Gene ID:55958
Uniprot:Q9P2J3
Cellular Location:
Calculated MW:69kDa
Observed MW:75kDa
Synonyms:KLHL9
Background:
UniProt Protein Function:KLHL9: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: midbody

Molecular Function:ubiquitin-protein ligase activity

Biological Process: mitosis; cytokinesis; protein ubiquitination

UniProt Code:Q9P2J3
NCBI GenInfo Identifier:51338828
NCBI Gene ID:55958
NCBI Accession:Q9P2J3.2
UniProt Secondary Accession:Q9P2J3,Q8TCQ2,
UniProt Related Accession:Q9P2J3
Molecular Weight:69,429 Da
NCBI Full Name:Kelch-like protein 9
NCBI Synonym Full Names:kelch-like family member 9
NCBI Official Symbol:KLHL9  
NCBI Protein Information:kelch-like protein 9; kelch-like 9
UniProt Protein Name:Kelch-like protein 9
Protein Family:Kelch-like protein
UniProt Gene Name:KLHL9  
UniProt Entry Name:KLHL9_HUMAN
Anti-KLHL9 Antibody (CAB10149)Western blot analysis of extracts of 293T cells, using KLHL9 antibody (CAB10149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-KLHL9 Antibody (CAB10149)Immunohistochemistry of paraffin-embedded rat ovary using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens).
Anti-KLHL9 Antibody (CAB10149)Immunohistochemistry of paraffin-embedded rat heart using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens).
Anti-KLHL9 Antibody (CAB10149)Immunohistochemistry of paraffin-embedded human breast cancer using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens).
Anti-KLHL9 Antibody (CAB10149)Immunohistochemistry of paraffin-embedded mouse testis using KLHL9 antibody (CAB10149) at dilution of 1:100 (40x lens).
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