Signal Transduction Antibodies 2

Anti-KLHL8 Antibody (CAB14917)

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SKU:
CAB14917
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-KLHL8 Antibody
Antibody SKU:CAB14917
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse brain, Mouse kidney, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AKEQ IVKQ NLKC RDLL DEAR NYHL HLSS RAVP DFEY SIRT TPRK HTAG VLFC VGGR GGSG DPFR SIEC YSIN KNSW FFGP EMNS RRRH VGVI SVEG KVYA VGGH DGNE HLGS MEMF DPLT NKWM MKAS MNTK RRGI ALAS LGGP IYAI GGLD DNTC FNDV ERYD IESD QWST VAPM NTPR GGVG SVAL VNHV YAVG GNDG MASL SSVE RYDP HLDK WIEV KEMG QRRA GNGV SKLH GCLY VVGG FDDN SPLS SVER YDPR SNKW DYVA ALTT PRGG VGIA TVMG KIFA VGGH NGNA YLNT VEAF DPVL NRWE LVGS VSHC RAGA GVAV CSCL TSQI RDVG HGSN NVVD CM
Gene ID:57563
Uniprot:Q9P2G9
Cellular Location:
Calculated MW:60kDa/68kDa
Observed MW:69kDa
Synonyms:KLHL8
Background:
UniProt Protein Function:KLHL8: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN. Component of the BCR(KLHL8) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL8 and RBX1. Interacts with RAPSN
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: nucleoplasm

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination

UniProt Code:Q9P2G9
NCBI GenInfo Identifier:635172825
NCBI Gene ID:57563
NCBI Accession:NP_001278932.1
UniProt Related Accession:Q9P2G9
Molecular Weight:60kDa; 68kDa
NCBI Full Name:kelch-like protein 8 isoform 1
NCBI Synonym Full Names:kelch like family member 8
NCBI Official Symbol:KLHL8  
NCBI Protein Information:kelch-like protein 8
UniProt Protein Name:Kelch-like protein 8
Protein Family:Kelch-like protein
UniProt Gene Name:KLHL8  
UniProt Entry Name:KLHL8_HUMAN
Anti-KLHL8 Antibody (CAB14917)Western blot analysis of extracts of various cell lines, using KLHL8 antibody (CAB14917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-KLHL8 Antibody (CAB14917)Immunofluorescence analysis of C6 cells using KLHL8 antibody (CAB14917) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-KLHL8 Antibody (CAB14917)Immunofluorescence analysis of L929 cells using KLHL8 antibody (CAB14917) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-KLHL8 Antibody (CAB14917)Immunofluorescence analysis of U-2 OS cells using KLHL8 antibody (CAB14917) at dilution of 1:100. Blue: DAPI for nuclear staining.
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