Cell Biology Antibodies 11
Anti-KLF9 Antibody (CAB7196)
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- SKU:
- CAB7196
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-KLF9 Antibody |
| Antibody SKU: | CAB7196 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human KLF9 (NP_001197.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, K-562, HepG2, Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human KLF9 (NP_001197.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAA AYMD FVAA QCLV SISN RAAV PEHG VAPD AERL RLPE REVT KEHG DPGD TWKD YCTL VTIA KSLL DLNK YRPI QTPS VCSD SLES PDED MGSD SDVT TESG SSPS HSPE ERQD PGSA PSPL SLLH PGVA AKGK HASE KRHK CPYS GC |
| Gene ID: | 687 |
| Uniprot: | Q13886 |
| Cellular Location: | Nucleus |
| Calculated MW: | 27kDa |
| Observed MW: | 30kDa |
| Synonyms: | KLF9, BTEB, BTEB1 |
| Background: | The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. |
| UniProt Protein Function: | KLF9: Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. Belongs to the Sp1 C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding Chromosomal Location of Human Ortholog: 9q13 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus Molecular Function:DNA binding; metal ion binding; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; circadian rhythm; progesterone receptor signaling pathway; transcription, DNA-dependent; embryo implantation |
| NCBI Summary: | The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13886 |
| NCBI GenInfo Identifier: | 3913177 |
| NCBI Gene ID: | 687 |
| NCBI Accession: | Q13886.1 |
| UniProt Secondary Accession: | Q13886,Q16196, B2R943, |
| UniProt Related Accession: | Q13886 |
| Molecular Weight: | 244 |
| NCBI Full Name: | Krueppel-like factor 9 |
| NCBI Synonym Full Names: | Kruppel-like factor 9 |
| NCBI Official Symbol: | KLF9 |
| NCBI Official Synonym Symbols: | BTEB; BTEB1 |
| NCBI Protein Information: | Krueppel-like factor 9; BTE-binding protein 1; GC-box-binding protein 1; transcription factor BTEB1; basic transcription element binding protein 1; basic transcription element-binding protein 1 |
| UniProt Protein Name: | Krueppel-like factor 9 |
| UniProt Synonym Protein Names: | Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Transcription factor BTEB1 |
| Protein Family: | Krueppel-like factor |
| UniProt Gene Name: | KLF9 |
| UniProt Entry Name: | KLF9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KLF9 antibody (CAB7196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of A-549 cells using KLF9 antibody (CAB7196). Blue: DAPI for nuclear staining. |
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