Epigenetics & Nuclear Signaling Antibodies 2

Anti-KLF8 Antibody (CAB16321)

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SKU:
CAB16321
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-KLF8 Antibody
Antibody SKU:CAB16321
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 210-280 of human KLF8 (NP_009181.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, 293T, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 210-280 of human KLF8 (NP_009181.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GKNA GSVK VDPT SMSP LEIP SDSE ESTI ESGS SALQ SLQG LQQE PAAM AQMQ GEES LDLK RRRI HQCD FAG
Gene ID:11279
Uniprot:O95600
Cellular Location:
Calculated MW:26kDa/27kDa/30kDa/39kDa
Observed MW:45kDa
Synonyms:KLF8, BKLF3, ZNF741
Background:This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:KLF8: Transcriptional repressor and activator. Binds to CACCC- boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). Belongs to the Sp1 C2H2-type zinc-finger protein family.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: nucleus

Molecular Function:DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Code:O95600
NCBI GenInfo Identifier:17366251
NCBI Gene ID:11279
NCBI Accession:O95600.2
UniProt Related Accession:O95600
Molecular Weight:Calculated: 26kDa; 27kDa; 30kDa; 39kDaObserved: 45kDa
NCBI Full Name:Krueppel-like factor 8
NCBI Synonym Full Names:Kruppel like factor 8
NCBI Official Symbol:KLF8  
NCBI Official Synonym Symbols:BKLF3; ZNF741  
NCBI Protein Information:Krueppel-like factor 8
UniProt Protein Name:Krueppel-like factor 8
UniProt Synonym Protein Names:Basic krueppel-like factor 3; Zinc finger protein 741
Protein Family:Krueppel-like factor
UniProt Gene Name:KLF8  
UniProt Entry Name:KLF8_HUMAN
Anti-KLF8 Antibody (CAB16321)Western blot analysis of extracts of various cell lines, using KLF8 antibody (CAB16321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-KLF8 Antibody (CAB16321)Immunofluorescence analysis of A431 cells using KLF8 antibody (CAB16321) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-KLF8 Antibody (CAB16321)Immunofluorescence analysis of L929 cells using KLF8 antibody (CAB16321) at dilution of 1:100. Blue: DAPI for nuclear staining.
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