Epigenetics & Nuclear Signaling Antibodies 3

Anti-KLF5 Antibody (CAB2989)

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SKU:
CAB2989
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-KLF5 Antibody
Antibody SKU:CAB2989
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLF5 (NP_001721.2).
Application:WB IHC IF
Recommended Dilution:WB 1:200 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:U-87MG, HeLa, A-549, HT-1080, HT-29
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLF5 (NP_001721.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATR VLSM SARL GPVP QPPA PQDE PVFA QLKP VLGA ANPA RDAA LFPG EELK HAHH RPQA QPAP AQAP QPAQ PPAT GPRL PPED LVQT RCEM EKYL TPQL PPVP IIPE HKKY RRDS ASVV DQFF TDTE GLPY SINM NVFL PDIT HLRT GLYK SQRP CVTH IKTE PVAI FSHQ SETT APPP APTQ ALPE FTSI FSSH QTAA PEVN NIFI KQEL PTPD LHLS VPTQ QGHL YQLL NTPD LDMP SSTN QTAA MD
Gene ID:688
Uniprot:Q13887
Cellular Location:Nucleus
Calculated MW:18kDa/33kDa/41kDa/50kDa
Observed MW:55kDa
Synonyms:KLF5, BTEB2, CKLF, IKLF
Background:This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:KLF5: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; plasma membrane

Molecular Function:protein binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; microvillus biogenesis; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of microvillus biogenesis

NCBI Summary:This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
UniProt Code:Q13887
NCBI GenInfo Identifier:12644412
NCBI Gene ID:688
NCBI Accession:Q13887.2
UniProt Secondary Accession:Q13887,Q9UHP8, L0R3U5, L0R4T9,
UniProt Related Accession:Q13887
Molecular Weight:457
NCBI Full Name:Krueppel-like factor 5
NCBI Synonym Full Names:Kruppel-like factor 5 (intestinal)
NCBI Official Symbol:KLF5  
NCBI Official Synonym Symbols:CKLF; IKLF; BTEB2  
NCBI Protein Information:Krueppel-like factor 5; Klf5C isoform; BTE-binding protein 2; GC box binding protein 2; colon kruppel-like factor; colon krueppel-like factor; transcription factor BTEB2; intestinal-enriched kruppel-like factor; intestinal-enriched krueppel-like factor; b
UniProt Protein Name:Krueppel-like factor 5
UniProt Synonym Protein Names:Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2
Protein Family:Krueppel-like factor
UniProt Gene Name:KLF5  
UniProt Entry Name:KLF5_HUMAN
Anti-KLF5 Antibody (CAB2989)Western blot analysis of extracts of various cell lines, using KLF5 antibody (CAB2989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-KLF5 Antibody (CAB2989)Immunohistochemistry of paraffin-embedded human placenta using KLF5 antibody (CAB2989) at dilution of 1:100 (40x lens).
Anti-KLF5 Antibody (CAB2989)Immunofluorescence analysis of A375 cells using KLF5 antibody (CAB2989) at dilution of 1:100. Blue: DAPI for nuclear staining.
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