Epigenetics & Nuclear Signaling Antibodies 1
Anti-KLF1 Antibody (CAB10581)
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- SKU:
- CAB10581
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-KLF1 Antibody |
| Antibody SKU: | CAB10581 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLF1 (NP_006554.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | A-549, LO2, Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLF1 (NP_006554.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATA ETAL PSIS TLTA LGPF PDTQ DDFL KWWR SEEA QDMG PGPP DPTE PPLH VKSE DQPG EEED DERG ADAT WDLD LLLT NFSG PEPG GAPQ TCAL APSE |
| Gene ID: | 10661 |
| Uniprot: | Q13351 |
| Cellular Location: | Nucleus |
| Calculated MW: | 38kDa |
| Observed MW: | 38kDa |
| Synonyms: | KLF1, EKLF, CDAN4, HBFQTL6, INLU |
| Background: | This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. |
| UniProt Protein Function: | KLF1: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4). It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. Belongs to the krueppel C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19p13.13 Cellular Component: nuclear chromatin; nucleus Molecular Function:transcription factor activity Biological Process: erythrocyte differentiation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent Disease: Anemia, Congenital Dyserythropoietic, Type Iv; Blood Group--lutheran Inhibitor; Fetal Hemoglobin Quantitative Trait Locus 6 |
| NCBI Summary: | This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q13351 |
| NCBI GenInfo Identifier: | 2501699 |
| NCBI Gene ID: | 10661 |
| NCBI Accession: | Q13351.1 |
| UniProt Secondary Accession: | Q13351,Q6PIJ5, Q92899, |
| UniProt Related Accession: | Q13351 |
| Molecular Weight: | 38kDa |
| NCBI Full Name: | Krueppel-like factor 1 |
| NCBI Synonym Full Names: | Kruppel like factor 1 |
| NCBI Official Symbol: | KLF1 |
| NCBI Official Synonym Symbols: | EKLF |
| NCBI Protein Information: | Krueppel-like factor 1 |
| UniProt Protein Name: | Krueppel-like factor 1 |
| UniProt Synonym Protein Names: | Erythroid krueppel-like transcription factor; EKLF |
| Protein Family: | Krueppel-like factor |
| UniProt Gene Name: | KLF1 |
 | Western blot analysis of extracts of various cell lines, using KLF1 antibody (CAB10581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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