Cell Biology Antibodies 2

Anti-KL Antibody (CAB12028)

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SKU:
CAB12028
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KL Antibody
Antibody SKU:CAB12028
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KL (NP_004786.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:DU145, Mouse kidney, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KL (NP_004786.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAS APPR RPRP PPPS LSLL LVLL GLGG RRLR AEPG DGAQ TWAR FSRP PAPE AAGL FQGT FPDG FLWA VGSA AYQT EGGW QQHG KGAS IWDT FTHH PLAP PGDS RNAS LPLG APSP LQPA TGDV ASDS YNNV FRDT EALR ELGV THYR FSIS WARV LPNG SAGV PNRE GLRY YRRL LERL RELG VQPV VTLY HWDL PQRL
Gene ID:9365
Uniprot:Q9UEF7
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:62kDa/116kDa
Observed MW:65kDa, 120kDa
Synonyms:KL, klotho
Background:This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
UniProt Protein Function:Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Hydrolase; EC 3.2.1.31

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular region; integral to plasma membrane; plasma membrane

Molecular Function:1-phosphatidylinositol-3-kinase activity; beta-glucosidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: aging; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphoinositide-mediated signaling; positive regulation of bone mineralization; regulation of phosphoinositide 3-kinase cascade

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial

NCBI Summary:This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UEF7
NCBI GenInfo Identifier:77416517
NCBI Gene ID:9365
NCBI Accession:Q9UEF7.2
UniProt Secondary Accession:Q9UEF7,Q5VZ95, Q96KV5, Q96KW5, Q9UEI9, Q9Y4F0,
UniProt Related Accession:Q9UEF7
Molecular Weight:62,135 Da
NCBI Full Name:Klotho
NCBI Synonym Full Names:klotho
NCBI Official Symbol:KL  
NCBI Protein Information:klotho
UniProt Protein Name:Klotho
Protein Family:Klotho
UniProt Gene Name:KL  
UniProt Entry Name:KLOT_HUMAN
Anti-KL Antibody (CAB12028)Western blot analysis of extracts of various cell lines, using KL antibody (CAB12028) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-KL Antibody (CAB12028)Immunohistochemistry of paraffin-embedded human lung cancer using KL antibody (CAB12028) at dilution of 1:200 (40x lens).
Anti-KL Antibody (CAB12028)Immunohistochemistry of paraffin-embedded human placenta using KL antibody (CAB12028) at dilution of 1:200 (40x lens).
Anti-KL Antibody (CAB12028)Immunofluorescence analysis of rat kidney using KL antibody (CAB12028) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-KL Antibody (CAB12028)Immunofluorescence analysis of human placenta using KL antibody (CAB12028) at dilution of 1:100. Blue: DAPI for nuclear staining.
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