Cell Biology Antibodies 4
Anti-KIRREL Antibody (CAB14283)
- SKU:
- CAB14283
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-KIRREL Antibody |
| Antibody SKU: | CAB14283 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-200 of human KIRREL (NP_060710.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-200 of human KIRREL (NP_060710.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QGTQ TRFS QEPA DQTV VAGQ RAVL PCVL LNYS GIVQ WTKD GLAL GMGQ GLKA WPRY RVVG SADA GQYN LEIT DAEL SDDA SYEC QATE AALR SRRA KLTV LIPP EDTR IDGG PVIL LQAG TPHN LTCR AFNA KPAA TIIW FRDG TQQE GAVA STEL LKDG KRET TVSQ LLIN PTDL DIGR VFTC |
| Gene ID: | 55243 |
| Uniprot: | Q96J84 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 72kDa/83kDa/85kDa |
| Observed MW: | 83kDa |
| Synonyms: | KIRREL, NEPH1 |
| Background: |
| UniProt Protein Function: | Function: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1 |
| UniProt Protein Details: | By similarity. Subunit structure: Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2 By similarity. Subcellular location: Cell membrane; Single-pass type I membrane protein Potential. Note: Predominantly located at podocyte slit diaphragm. Tissue specificity: Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. Ref.1 Post-translational modification: Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding By similarity.N-glycosylated By similarity. Sequence similarities: Belongs to the immunoglobulin superfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Sequence caution: The sequence AAP59845.1 differs from that shown. Reason: Frameshift at position 19. The sequence BAA91850.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14192.1 differs from that shown. Reason: Erroneous initiation. |
| NCBI Summary: | NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96J84 |
| NCBI GenInfo Identifier: | 80478236 |
| NCBI Gene ID: | 55243 |
| NCBI Accession: | AAI09194.1 |
| UniProt Related Accession: | Q96J84 |
| Molecular Weight: | |
| NCBI Full Name: | KIRREL protein |
| NCBI Synonym Full Names: | kirre like nephrin family adhesion molecule 1 |
| NCBI Official Symbol: | KIRREL1 |
| NCBI Official Synonym Symbols: | NEPH1; KIRREL |
| NCBI Protein Information: | kin of IRRE-like protein 1 |
| UniProt Protein Name: | Kin of IRRE-like protein 1 |
| UniProt Synonym Protein Names: | Kin of irregular chiasm-like protein 1; Nephrin-like protein 1 |
| UniProt Gene Name: | KIRREL |
| UniProt Entry Name: | KIRR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIRREL antibody (CAB14283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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