Antibody Name:	Anti-KIR2DS4 Antibody
Antibody SKU:	CAB1779
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-245 of human KIR2DS4 (NP_036446.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-245 of human KIR2DS4 (NP_036446.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QEGV HRKP SFLA LPGH LVKS EETV ILQC WSDV MFEH FLLH REGK FNNT LHLI GEHH DGVS KANF SIGP MMPV LAGT YRCY GSVP HSPY QLSA PSDP LDMV IIGL YEKP SLSA QPGP TVQA GENV TLSC SSRS SYDM YHLS REGE AHER RLPA VRSI NGTF QADF PLGP ATHG GTYR CFGS FRDA PYEW SNSS DPLL VSVT GNPS NSWP SPTE PSSK TGNP RHLH
Gene ID:	3809
Uniprot:	P43632
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	33kDa
Observed MW:	35kDa

Synonyms:

KIR2DS4, CD158I, KIR-2DS4, KIR1D, KIR412, KKA3, NKAT-8, NKAT8

Background:

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several 'framework' genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.

UniProt Protein Function:	KIR2DS4: Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells. Belongs to the immunoglobulin superfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: integral to plasma membrane; plasma membrane Biological Process: innate immune response
NCBI Summary:	Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]
UniProt Code:	P43632
NCBI GenInfo Identifier:	2828511
NCBI Gene ID:	3809
NCBI Accession:	P43632.2
UniProt Secondary Accession:	P43632,Q6H2G7,
UniProt Related Accession:	P43632
Molecular Weight:	304
NCBI Full Name:	Killer cell immunoglobulin-like receptor 2DS4
NCBI Synonym Full Names:	killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
NCBI Official Symbol:	KIR2DS4
NCBI Official Synonym Symbols:	KKA3; KIR1D; NKAT8; CD158I; KIR412; NKAT-8; KIR2DS1
NCBI Protein Information:	killer cell immunoglobulin-like receptor 2DS4; KIR antigen 2DS4; killer Ig receptor; p58 NK receptor CL-39/CL-17; MHC class I NK cell receptor; killer inhibitory receptor 4-1-2; CD158 antigen-like family member I; natural killer-associated transcript 8; natural killer cell inhibitory receptor; P58 natural killer cell receptor clones CL-39/CL-17; killer cell immunoglobulin-like receptor two domains short cytoplasmic tail 1
UniProt Protein Name:	Killer cell immunoglobulin-like receptor 2DS4
UniProt Synonym Protein Names:	CD158 antigen-like family member I; MHC class I NK cell receptor; Natural killer-associated transcript 8; NKAT-8; P58 natural killer cell receptor clones CL-39/CL-17; p58 NK receptor CL-39/CL-17; CD_antigen: CD158i
Protein Family:	Killer cell immunoglobulin-like receptor
UniProt Gene Name:	KIR2DS4
UniProt Entry Name:	KI2S4_HUMAN

	Western blot analysis of extracts of 293T cells, using KIR2DS4 antibody (CAB1779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using KIR2DS4 antibody (CAB1779) at dilution of 1:100. Blue: DAPI for nuclear staining.