Cell Biology Antibodies 12

Anti-KIFC3 Antibody (CAB8617)

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SKU:
CAB8617
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KIFC3 Antibody
Antibody SKU:CAB8617
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-390 of human KIFC3 (NP_005541.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Rat
Positive Samples:U-87MG, DU145, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-390 of human KIFC3 (NP_005541.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVEN ERLR QEMR RCEA ELQE LRTK PAGP CPGC EHSQ ESAQ LRDK LSQL QLEM AESK GMLS ELNL EVQQ KTDR LAEV ELRL KDCL AEKA QEEE RLSR RLRD SHET IASL RAQS PPVK YVIK TVEV ESSK TKQA LSES QARN QHLQ EQVA MQRQ VLKE MEQQ LQSS HQLT ARLR AQIA MYES ELER AHGQ MLEE MQSL EEDK NRAI EEAF ARAQ VEMK AVHE NLAG VRTN LLTL QPAL RTLT NDYN GLKR QVR
Gene ID:3801
Uniprot:Q9BVG8
Cellular Location:Cell junction, Cytoplasm, Cytoplasmic vesicle membrane, Peripheral membrane protein, adherens junction, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:71kDa/77kDa/92kDa/94kDa
Observed MW:120kDa
Synonyms:KIFC3
Background:This gene encodes a member of the kinesin-14 family of microtubule motors. Members of this family play a role in the formation, maintenance and remodeling of the bipolar mitotic spindle. The protein encoded by this gene has cytoplasmic functions in the interphase cells. It may also be involved in the final stages of cytokinesis. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:KIFC3: Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance. Belongs to the kinesin-like protein family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 16q13-q21

Cellular Component: Golgi apparatus; microtubule; kinesin complex; centrosome; cytoplasmic vesicle membrane; zonula adherens

Molecular Function:microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: visual perception; metabolic process; zonula adherens maintenance; Golgi organization and biogenesis; microtubule-based movement

NCBI Summary:KIFC3 belongs to the large superfamily of kinesins, molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes, such as cell division, organelle transport, and nuclear movement (Hoang et al., 1998 [PubMed 9782090]).[supplied by OMIM, Mar 2009]
UniProt Code:Q9BVG8
NCBI GenInfo Identifier:194294508
NCBI Gene ID:3801
NCBI Accession:NP_001123571.1
UniProt Secondary Accession:Q9BVG8,O75299, Q49A29, Q49AQ0, Q59G19, Q8IUT3, Q96HW6 A8K6S2, B7Z484,
UniProt Related Accession:Q9BVG8
Molecular Weight:94,276 Da
NCBI Full Name:kinesin-like protein KIFC3 isoform 3
NCBI Synonym Full Names:kinesin family member C3
NCBI Official Symbol:KIFC3  
NCBI Protein Information:kinesin-like protein KIFC3
UniProt Protein Name:Kinesin-like protein KIFC3
Protein Family:Kinesin-like protein
UniProt Gene Name:KIFC3  
UniProt Entry Name:KIFC3_HUMAN
Anti-KIFC3 Polyclonal Antibody (CAB8617)Western blot analysis of extracts of various cell lines, using KIFC3 antibody (CAB8617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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