Cell Cycle Antibodies 1
Anti-KIFC1 Antibody (CAB3304)
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- SKU:
- CAB3304
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-KIFC1 Antibody |
| Antibody SKU: | CAB3304 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HT-29, HeLa, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDPQ RSPL LEVK GNIE LKRP LIKA PSQL PLSG SRLK RRPD QMED GLEP EKKR TRGL GATT KITT SHPR VPSL TTVP QTQG QTTA QKVS KKTG PRCS TAIA TGLK NQKP VPAV PVQK SGTS GVPP MAGG KKPS KRPA WDLK GQLC DLNA ELKR CRER TQTL DQEN QQLQ DQLR DAQQ QVKA LGTE RTTL EGHL AKVQ AQAE QGQQ ELKN LRAC VLEL EERL STQE GLVQ ELQK KQVE LQEE RRGL MSQL EE |
| Gene ID: | 3833 |
| Uniprot: | Q9BW19 |
| Cellular Location: | Cytoplasm, Early endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle |
| Calculated MW: | 73kDa |
| Observed MW: | 74kDa |
| Synonyms: | KIFC1, HSET, KNSL2 |
| Background: |
| UniProt Protein Function: | KIFC1: Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Belongs to the kinesin-like protein family. NCD subfamily. Binds NUBP1 and NUBP2. |
| UniProt Protein Details: | Protein type:Motor; Microtubule-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: kinesin complex; microtubule; membrane; early endosome; microtubule organizing center; spindle; nucleus Molecular Function:minus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding Biological Process: mitotic sister chromatid segregation; metabolic process; cell division; spindle assembly; blood coagulation; mitotic metaphase plate congression; microtubule-based movement |
| UniProt Code: | Q9BW19 |
| NCBI GenInfo Identifier: | 20138710 |
| NCBI Gene ID: | 3833 |
| NCBI Accession: | Q9BW19.2 |
| UniProt Secondary Accession: | Q9BW19,O60887, Q14834, Q4KMP0, Q5SU09, Q6GMS7, Q6P4A5 Q9UQP7, |
| UniProt Related Accession: | Q9BW19 |
| Molecular Weight: | 74kDa |
| NCBI Full Name: | Kinesin-like protein KIFC1 |
| NCBI Synonym Full Names: | kinesin family member C1 |
| NCBI Official Symbol: | KIFC1 |
| NCBI Official Synonym Symbols: | HSET; KNSL2 |
| NCBI Protein Information: | kinesin-like protein KIFC1; kinesin-like 2; kinesin-like protein 2; kinesin-related protein HSET |
| UniProt Protein Name: | Kinesin-like protein KIFC1 |
| UniProt Synonym Protein Names: | Kinesin-like protein 2; Kinesin-related protein HSET |
| Protein Family: | Kinesin-like protein |
| UniProt Gene Name: | KIFC1 |
| UniProt Entry Name: | KIFC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIFC1 antibody (CAB3304) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat testis using KIFC1 antibody (CAB3304) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using KIFC1 antibody (CAB3304) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using KIFC1 antibody (CAB3304) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using KIFC1 Polyclonal Antibody (CAB3304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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